Item Details

Targeting Aerobic Glycolysis Cancer at the Level of Phosphofructo-1-Kinase

Kefauver, Jennifer
Format
Thesis/Dissertation; Online
Author
Kefauver, Jennifer
Abstract
Aerobic glycolysis is a hallmark of proliferating cells; in cancer cells, it is the predominant metabolic profile, providing tumors benefits for growth and metastasis. The allosteric enzyme phosphofructokinase-1 (PFK-1) catalyzes the rate-limiting step of glycolysis. In tumor cells, the liver (L) isoform of this enzyme is predominantly expressed over the other isoforms at both the mRNA and protein levels. Our lab seeks to inhibit the PFKL enzyme in order to arrest glycolytic flux, which may reduce growth and survival of tumor cells. It has been shown that plasmids containing short hairpin (sh)RNA constructs that bind to PFKL mRNA have reduced the expression of the PFKL protein. This tool can be used to study the effect of PFKL knockdown and determine the value of designing a specific PFKL protein inhibitor. Note: Abstract extracted from PDF text
Language
English
Date Received
20140123
Published
University of Virginia, Department of Biology, MA (Master of Arts), 2012
Published Date
2012-07-01
Degree
MA (Master of Arts)
Collection
Libra ETD Repository
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