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A Novel Label-Free Modality for DNA Quantification and Cell Counting Towards Clinical Analysis in Microfluidic Systems

Li, Jingyi
Format
Thesis/Dissertation; Online
Author
Li, Jingyi
Advisor
Venton, Jill
Columbus, Linda
Cafiso, David
Abstract
The advance in microfluidics has enabled various novel analyses in biomedical studies, with advantages such as improved portability and reduced reagent consumption. However, challenges remain in this field, as the assay complexity generally increases with reduced dimensions of instruments, while simplicity and cost-effectiveness are desired for prevalent applications. As a result, efforts are required to tailor convectional methods, and in some cases, to invent new approaches, for microfluidic platforms. DNA quantification and cell counting are two keystones in biological and medical sciences. Conventional technologies requires large and expensive footprint, and thus are not suitable for integration in microfluidic systems. This dissertation presents a new approach, coined as 'the pinwheel assay', to quantify DNA and to enumerate cells via the DNA-induced aggregation of paramagnetic particles in an engineered magnetic field, which can be readily adapted for microfluidic applications. Note: Abstract extracted from PDF text
Language
English
Published
University of Virginia, Department of Chemistry, PHD (Doctor of Philosophy), 2012
Published Date
2012-12-01
Degree
PHD (Doctor of Philosophy)
Collection
Libra ETD Repository
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