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Multiple Sclerosis [electronic resource]: Methods and Protocols

edited by Robert Weissert, University of Regensburg, Regensburg, Germany
Format
EBook; Book; Online
Published
New York : Humana Press, [2016]
Language
English
Series
Methods in Molecular Biology
Springer Protocols
Methods in Molecular Biology (Clifton, N.J.)
Springer Protocols (Series)
ISBN
9781493926299, 1493926292, 9781493926305, 1493926306
Summary
"This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models. With so much effort needed to elucidate basic disease mechanisms, to clone disease relevant genes, to define novel biomarkers, and to discover novel and improved therapeutic and curative treatments, this book serves to aid researchers in accomplishing these enormous goals. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Multiple Sclerosis: Methods and Protocols will empower the reader to perform novel research regarding pathophysiology and treatment for MS."--
Contents
  • Part I Molecular Biology Techniques
  • Positional Gene Cloning in Experimental Populations / Maja Jagodic and Pernilla Stridh
  • Generation of Transgenic Rats Using Lentiviral Vectors / Holger M. Reichard and Henrike J. Fischer
  • DNA Vaccination Techniques / Nicolás Fissolo, Xavier Montalban, and Manuel Comabella
  • Part II Cellular Techniques
  • Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture / Christine Riedhammer, Dagmar Halbritter, and Robert Weissert
  • ELISPOT Techniques / Niannian Ji and Thomas G. Forsthuber
  • Isolation of Central Nervous System (CNS) Infiltrating Cells / Ilgiz A. Mufazalov and Ari Waisman
  • Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System / Darryl G. Turner [and three others]
  • Part III Biomarker Techniques
  • Neurofilament Light Chain Determination from Peripheral Blood Samples / Marguerite Limberg [and three others]
  • Detection of Autoantibodies Against Myelin Oligodendrocyte Glycoprotein in Multiple Sclerosis and Related Diseases / Melania Spadaro and Edgar Meinl
  • Myelinating Cultures: An In Vitro Tool to Identify Demyelinating and Axopathic Autoantibodies / Maren Lindner and Christopher Linington
  • Part IV Imaging Techniques
  • In Vivo Visualization of (Auto- ) Immune Processes in the Central Nervous System of Rodents / Christian Schlä̈ger [and three others]
  • Optical Coherence Tomography to Assess Neurodegeneration in Multiple Sclerosis / Axel Petzold
  • Part V Disease Models
  • Experimental Autoimmune Encephalomyelitis in Mice / Rachael L. Terry, Igal Ifergan, and Stephen D. Miller
  • Actively Induced Experimental Autoimmune Encephalomyelitis in Rats / Robert Weissert
  • Experimental Autoimmune Encephalomyelitis in Marmosets / S. Anwar Jagessar [and four others]
  • Part VI Neuropathological Techniques
  • Neuropathological Techniques to Investigate CNS Pathology in Experimental Autoimmune Encephalomyelitis (EAE) / Karin Steinbach and Doron Merkler
  • Neuropathological Techniques to Investigate Central Nervous System Sections in Multiple Sclerosis / Jan Bauer and Hans Lassmann.
Description
Mode of access: World wide Web.
Notes
  • "ISSN 1940-6029 (electronic)"--Title page verso.
  • Includes bibliographical references and index.
Series Statement
Methods in molecular biology, 1064-3745 ; 1304
Methods in molecular biology (Clifton, N.J.) ; v. 1304
Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
  • Access in Virgo Classic
  • Staff View

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    a| "ISSN 1940-6029 (electronic)"--Title page verso.
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    g| Part I Molecular Biology Techniques -- t| Positional Gene Cloning in Experimental Populations / r| Maja Jagodic and Pernilla Stridh -- t| Generation of Transgenic Rats Using Lentiviral Vectors / r| Holger M. Reichard and Henrike J. Fischer -- t| DNA Vaccination Techniques / r| Nicolás Fissolo, Xavier Montalban, and Manuel Comabella -- g| Part II Cellular Techniques -- t| Peripheral Blood Mononuclear Cells: Isolation, Freezing, Thawing, and Culture / r| Christine Riedhammer, Dagmar Halbritter, and Robert Weissert -- t| ELISPOT Techniques / r| Niannian Ji and Thomas G. Forsthuber -- t| Isolation of Central Nervous System (CNS) Infiltrating Cells / r| Ilgiz A. Mufazalov and Ari Waisman -- t| Methods for Ex Vivo Analysis of Immune Cell Function from the Central Nervous System / r| Darryl G. Turner [and three others] -- g| Part III Biomarker Techniques -- t| Neurofilament Light Chain Determination from Peripheral Blood Samples / r| Marguerite Limberg [and three others] -- t| Detection of Autoantibodies Against Myelin Oligodendrocyte Glycoprotein in Multiple Sclerosis and Related Diseases / r| Melania Spadaro and Edgar Meinl -- t| Myelinating Cultures: An In Vitro Tool to Identify Demyelinating and Axopathic Autoantibodies / r| Maren Lindner and Christopher Linington -- g| Part IV Imaging Techniques -- t| In Vivo Visualization of (Auto- ) Immune Processes in the Central Nervous System of Rodents / r| Christian Schlä̈ger [and three others] -- t| Optical Coherence Tomography to Assess Neurodegeneration in Multiple Sclerosis / r| Axel Petzold -- g| Part V Disease Models -- t| Experimental Autoimmune Encephalomyelitis in Mice / r| Rachael L. Terry, Igal Ifergan, and Stephen D. Miller -- t| Actively Induced Experimental Autoimmune Encephalomyelitis in Rats / r| Robert Weissert -- t| Experimental Autoimmune Encephalomyelitis in Marmosets / r| S. Anwar Jagessar [and four others] -- g| Part VI Neuropathological Techniques -- t| Neuropathological Techniques to Investigate CNS Pathology in Experimental Autoimmune Encephalomyelitis (EAE) / r| Karin Steinbach and Doron Merkler -- t| Neuropathological Techniques to Investigate Central Nervous System Sections in Multiple Sclerosis / r| Jan Bauer and Hans Lassmann.
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    a| "This detailed volume covers molecular biology, cellular biology, biomarkers, imaging, and neuropathological methods and techniques to explore multiple sclerosis (MS), with a special emphasis on disease models. With so much effort needed to elucidate basic disease mechanisms, to clone disease relevant genes, to define novel biomarkers, and to discover novel and improved therapeutic and curative treatments, this book serves to aid researchers in accomplishing these enormous goals. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Multiple Sclerosis: Methods and Protocols will empower the reader to perform novel research regarding pathophysiology and treatment for MS."-- c| Back cover.
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    a| Mode of access: World wide Web.
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    a| Multiple sclerosis x| Research v| Laboratory manuals.
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    a| Springer Science+Business Media.
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    a| Springer Protocols
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    a| Methods in molecular biology (Clifton, N.J.) ; v| v. 1304.
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