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T-Cell Development [electronic resource]: Methods and Protocols

edited by Rémy Bosselut and Melanie S. Vacchio, Laboratory of Immune Cell biology, National Institutes of Health, National Cancer Institute, Bethesda, MD, USA
Format
EBook; Book; Online
Published
New York : Humana Press, [2015]
Language
English
Series
Methods in Molecular Biology
Springer Protocols
Methods in Molecular Biology
Springer Protocols (Series)
ISBN
9781493928088, 1493928082, 9781493928095, 1493928090
Contents
  • 200 million thymocytes and I : a beginner's survival guide to T cell development / Melanie S. Vacchio, Thomas Ciucci, and Rémy Bosselut
  • Development of [gamma] [delta] T cells, the special-force soldiers of the immune system / David L. Wiest
  • Genetic tools to study T cell development by flow cytometry / Thomas Ciucci, Melanie S. Vacchio, and Rémy Bosselut
  • Assessment of T cell development by flow cytometry / Jan Y.M. Lee and Paul E. Love
  • Flow cytometry analysis of thymic epithelial cells and their subpopulations / Izumi Ohigashi and Yousuke Takahama
  • Identifying the spatial relationships of thymic stromal and thymocyte subsets by immunofluorescence analysis / Virgnia Bain and Ellen R. Richie
  • Purification of thymocyte and T cell subsets / Andrea C. Carpenter, Jong Kyong Kim, and Rémy Bosselut
  • Retroviral transduction of T cells and T cell precursors / Amie Simmons and José Alberola-Ila
  • Bone marrow and fetal liver radiation chimeras / Francis A. Flomerfelt and Ronald E. Gress
  • In vitro analyses of T cell effector differentiation / Elizabeth A. Wohlfert [and three others]
  • Studying T cell development in thymic slices / Jenny O. Ross [and three others]
  • FTOC-based analysis of negative selection / Cody A. Cunningham, Emma Teixeiro, and Mark A. Daniels
  • Reconstituted thymus organ culture / Zimu Deng [and three others]
  • Induction of T cell development in virto by delta-like (Dll)-expressing stromal cells / Mahmood Mohtashami, Payam Zarin, and Juan Carlos Zúñiga-Pflücker
  • In vitro analysis of thymocyte signaling / Cody A. Cunningham, Emma Teixeiro, and Mark A. Daniels
  • Molecular analysis of mouse T cell receptor [alpha] and [beta] gene rearrangements / Levi J. Rupp [and three others]
  • Intrathymic injection / Sugata Manna and Avinash Bhandoola
  • Analysis of cell proliferation and homeostasis using EdU labeling / Francis A. Flomerfelt and Ronald E. Gress
  • Chracterization and isolation of human T cell progenitors / Inge Van de Walle, Karin Davids, and Tom Taghon
  • Approaches to study human T cell development / Anne-Catherine Dolens, Inge Van de Walle, and Tom Taghon
  • Humanized mice to study human T cell development / Sarah Bonte [and six others]
  • Using the Zebrafish model to study T cell development / Yong Zhang and David L. Wiest.
Description
Mode of access: World wide Web.
Notes
Includes bibliographical references and index.
Series Statement
Methods in Molecular Biology, 1064-3745 ; 1323
Methods in molecular biology ; v. 1323
Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
  • Access in Virgo Classic
  • Staff View

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    t| 200 million thymocytes and I : a beginner's survival guide to T cell development / r| Melanie S. Vacchio, Thomas Ciucci, and Rémy Bosselut -- t| Development of [gamma] [delta] T cells, the special-force soldiers of the immune system / r| David L. Wiest -- t| Genetic tools to study T cell development by flow cytometry / r| Thomas Ciucci, Melanie S. Vacchio, and Rémy Bosselut -- t| Assessment of T cell development by flow cytometry / r| Jan Y.M. Lee and Paul E. Love -- t| Flow cytometry analysis of thymic epithelial cells and their subpopulations / r| Izumi Ohigashi and Yousuke Takahama -- t| Identifying the spatial relationships of thymic stromal and thymocyte subsets by immunofluorescence analysis / r| Virgnia Bain and Ellen R. Richie -- t| Purification of thymocyte and T cell subsets / r| Andrea C. Carpenter, Jong Kyong Kim, and Rémy Bosselut -- t| Retroviral transduction of T cells and T cell precursors / r| Amie Simmons and José Alberola-Ila -- t| Bone marrow and fetal liver radiation chimeras / r| Francis A. Flomerfelt and Ronald E. Gress -- t| In vitro analyses of T cell effector differentiation / r| Elizabeth A. Wohlfert [and three others] -- t| Studying T cell development in thymic slices / r| Jenny O. Ross [and three others] -- t| FTOC-based analysis of negative selection / r| Cody A. Cunningham, Emma Teixeiro, and Mark A. Daniels -- t| Reconstituted thymus organ culture / r| Zimu Deng [and three others] -- t| Induction of T cell development in virto by delta-like (Dll)-expressing stromal cells / r| Mahmood Mohtashami, Payam Zarin, and Juan Carlos Zúñiga-Pflücker -- t| In vitro analysis of thymocyte signaling / r| Cody A. Cunningham, Emma Teixeiro, and Mark A. Daniels -- t| Molecular analysis of mouse T cell receptor [alpha] and [beta] gene rearrangements / r| Levi J. Rupp [and three others] -- t| Intrathymic injection / r| Sugata Manna and Avinash Bhandoola -- t| Analysis of cell proliferation and homeostasis using EdU labeling / r| Francis A. Flomerfelt and Ronald E. Gress -- t| Chracterization and isolation of human T cell progenitors / r| Inge Van de Walle, Karin Davids, and Tom Taghon -- t| Approaches to study human T cell development / r| Anne-Catherine Dolens, Inge Van de Walle, and Tom Taghon -- t| Humanized mice to study human T cell development / r| Sarah Bonte [and six others] -- t| Using the Zebrafish model to study T cell development / r| Yong Zhang and David L. Wiest.
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