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Recombinant Proteins From Plants [electronic resource]: Methods and Protocols

edited by Jacqueline MacDonald, London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada, Igor Kolotilin, Department of Biology, Western University, London, ON, Canada, Rima Menassa, London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada
Format
EBook; Book; Online
Published
New York : Humana Press, [2016]
Edition
Second edition
Language
English
Series
Methods in Molecular Biology
Springer Protocols
Methods in Molecular Biology (Clifton, N.J.)
Springer Protocols (Series)
ISBN
9781493932887, 1493932888, 9781493932894
Summary
This volume provides up-to-date scientific achievements from the world's top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming--
Contents
  • Chloroplast-based expression of recombinant proteins by Gateway® cloning technology / r Johanna Gottschamel and Andreas Lössl
  • Multigene engineering in rice using high-capacity Agrobacterium tumefaciens BIBAC vectors / Ruifeng He
  • Virus-derived vectors for the expression of multiple proteins in plants / Pooja Saxena [and others]
  • Transient protein expression by agroinfiltration in lettuce / Qiang Chen [and others]
  • Production of recombinant proteins in the chloroplast of the green alga Chlamydomonas reinhardtii / Daniel Guzmán-Zapata [and others]
  • Efficient, antibiotic marker-free transformation of a dicot and a monocot crop with glutamate 1-semialdehyde aminotransferase selectable marker genes / Nicoletta Ferradini [and others]
  • Transient expression of mammalian genes in N. benthamiana to modulate N-glycosylation / Alexandra Castilho and Herta Steinkellner
  • Companion protease inhibitors for the in situ protection of recombinant proteins in plants / Stéphanie Robert [and others]
  • Production of recombinant cholera toxin B subunit in Nicotiana benthamiana using GENEWARE® tobacco mosaic virus vector / Lauren Moore, Krystal Hamorsky, and Nobuyuki Matoba
  • Total soluble protein extraction for improved proteomic analysis of transgenic rice plant roots / Manish L. Raorane, Joan O. Narciso, and Ajay Kohli
  • Temporary immersion bioreactors for the contained production of recombinant proteins in transplastomic plants / Sherwin Barretto, Franck Michoux, and Peter J. Nixon
  • Plant cell-based recombinant antibody manufacturing with a 200 L orbitally shaken disposable bioreactor / Nicole Raven, Stefan Schillberg, and Stefan Rasche
  • Liquid-liquid phase separation of oil bodies from seeds / Cory L. Nykiforuk
  • Continuous flow separation of hydrophobin fusion proteins from plant cell culture extract / Lauri J. Reuter, Andrew J. Conley, and Jussi J. Joensuu
  • Molecular analyses of transgenic plants / Kurniawan Rudi Trijatmiko [and others]
  • Analysis of recombinant proteins in transgenic rice seeds : identity, localization, tolerance to digestion, and plant stress response / Yuhya Wakasa and Fumio Takaiwa
  • Real-time PCR-based quantitation method for the genetically modified soybean line GTS 40-3-2 / Kazumi Kitta, Reona Takabatake, and Junichi Mano
  • Standardized lepidopteran bioassay to investigate the bioactivity of insecticidal proteins produced in transgenic crops / Gerson Graser and Frederick S. Walters
  • Antibody-mediated pathogen resistance in plants / Dieter Peschen, Stefan Schillberg, and Rainer Fischer.
Description
Mode of access: World wide Web.
Notes
Includes bibliographical references and index.
Series Statement
Methods in molecular biology, 1064-3745 ; 1385
Methods in molecular biology (Clifton, N.J.) ; v. 1385. 1064-3745
Springer protocols (Series) 1949-2448
Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
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    a| Recombinant proteins from plants h| [electronic resource] : b| methods and protocols / c| edited by Jacqueline MacDonald, London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada, Igor Kolotilin, Department of Biology, Western University, London, ON, Canada, Rima Menassa, London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada.
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    a| New York : b| Humana Press, c| [2016]
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    a| Springer protocols
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    a| Includes bibliographical references and index.
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    t| Chloroplast-based expression of recombinant proteins by Gateway® cloning technology / t| r Johanna Gottschamel and Andreas Lössl -- t| Multigene engineering in rice using high-capacity Agrobacterium tumefaciens BIBAC vectors / r| Ruifeng He -- t| Virus-derived vectors for the expression of multiple proteins in plants / r| Pooja Saxena [and others] -- t| Transient protein expression by agroinfiltration in lettuce / r| Qiang Chen [and others] -- t| Production of recombinant proteins in the chloroplast of the green alga Chlamydomonas reinhardtii / r| Daniel Guzmán-Zapata [and others] -- t| Efficient, antibiotic marker-free transformation of a dicot and a monocot crop with glutamate 1-semialdehyde aminotransferase selectable marker genes / r| Nicoletta Ferradini [and others] -- t| Transient expression of mammalian genes in N. benthamiana to modulate N-glycosylation / r| Alexandra Castilho and Herta Steinkellner -- t| Companion protease inhibitors for the in situ protection of recombinant proteins in plants / r| Stéphanie Robert [and others] -- t| Production of recombinant cholera toxin B subunit in Nicotiana benthamiana using GENEWARE® tobacco mosaic virus vector / r| Lauren Moore, Krystal Hamorsky, and Nobuyuki Matoba -- t| Total soluble protein extraction for improved proteomic analysis of transgenic rice plant roots / r| Manish L. Raorane, Joan O. Narciso, and Ajay Kohli -- t| Temporary immersion bioreactors for the contained production of recombinant proteins in transplastomic plants / r| Sherwin Barretto, Franck Michoux, and Peter J. Nixon -- t| Plant cell-based recombinant antibody manufacturing with a 200 L orbitally shaken disposable bioreactor / r| Nicole Raven, Stefan Schillberg, and Stefan Rasche -- t| Liquid-liquid phase separation of oil bodies from seeds / r| Cory L. Nykiforuk -- t| Continuous flow separation of hydrophobin fusion proteins from plant cell culture extract / r| Lauri J. Reuter, Andrew J. Conley, and Jussi J. Joensuu -- t| Molecular analyses of transgenic plants / r| Kurniawan Rudi Trijatmiko [and others] -- t| Analysis of recombinant proteins in transgenic rice seeds : identity, localization, tolerance to digestion, and plant stress response / r| Yuhya Wakasa and Fumio Takaiwa -- t| Real-time PCR-based quantitation method for the genetically modified soybean line GTS 40-3-2 / r| Kazumi Kitta, Reona Takabatake, and Junichi Mano -- t| Standardized lepidopteran bioassay to investigate the bioactivity of insecticidal proteins produced in transgenic crops / r| Gerson Graser and Frederick S. Walters -- t| Antibody-mediated pathogen resistance in plants / r| Dieter Peschen, Stefan Schillberg, and Rainer Fischer.
    520
      
      
    a| This volume provides up-to-date scientific achievements from the world's top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming-- c| Source other than the Library of Congress.
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    a| Mode of access: World wide Web.
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    a| Recombinant proteins.
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    a| Plant genetic engineering.
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    a| Plant gene expression.
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    a| Electronic books.
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    a| MacDonald, Jacqueline c| (Of Agriculture and Agri-Food Canada)
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    a| Kolotilin, Igor.
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    a| Menassa, Rima.
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