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Estrogen Receptors [electronic resource]: Methods and Protocols

edited by Kathleen M. Eyster, University of South Dakota Sanford School of Medicine, Vermillion, South Dakota, USA
Format
EBook; Book; Online
Published
New York : Humana Press, [2016]
Language
English
Series
Methods in Molecular Biology
Springer Protocols
Methods in Molecular Biology (Clifton, N.J.)
Springer Protocols (Series)
ISBN
9781493931262 (hbk : acid-free paper), 1493931261 (hbk : acid-free paper), 9781493931279 (eBook), 149393127X (eBook)
Summary
This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen--
Contents
  • The estrogen receptors : an overview from different perspectives / Kathleen M. Eyster
  • Competitive binding assay for the G-protein-coupled receptor 30 (GPR30) or G-protein-coupled estrogen receptor (GPER) / Thomas Thekkumkara, Russell Snyder, and Vardan T. Karamyan
  • he use of real-time reverse transcription-PCR for assessing estrogen receptor and estrogen-responsive gene expression / Michelle L. Booze and Kathleen M. Eyster
  • Bioinformatics analysis of estrogen-responsive genes / Adam E. Handel
  • Electrophoretic mobility shift assay (EMSA) and supershift assay of cytochrome P450 2B6 in response to estrogen / Kwi Hye Koh and Hyunyoung Jeong
  • Chromatin immunoprecipitation assay to identify genomic binding sites of regulatory factors / Meike Wagner [and five others]
  • Chromatin immunoprecipitation with estrogen receptor 1 and the promoter of Greb1, in TM4 Sertoli cells / Jing Lin and Zhenmin Lei
  • Chromatin immunoprecipitation-sequencing (ChIP-seq) for mapping of estrogen receptor-chromatin interactions in breast cancer / Kelly A. Holmes, Gordon D. Brown, and Jason S. Carroll
  • RNA-Seq experiment and data analysis / Hanquan Liang and Erliang Zeng
  • DNA microarray analysis of estrogen-responsive genes / Kathleen M. Eyster
  • Shotgun proteomics analysis of estrogen effects in the uterus using two-dimensional liquid chromatography and tandem mass spectrometry / Eduardo A. Callegari
  • Assessment of protein expression by proximity ligation assay in the nonhuman primate endometrium, placenta, and fetal adrenal in response to estrogen / Thomas W. Bonagura [and three others]
  • Colocalization of estrogen receptors with the fluorescent tamoxifen derivative, FLTX1, analyzed by confocal microscopy / Araceli Morales [and four others]
  • Live-cell imaging of the estrogen receptor by total internal reflection fluorescence microscopy / Kassandra Kisler and Reymundo Dominguez
  • In situ hybridization of estrogen receptors [alpha] and [beta] and GPER in the human testis / Daniela Fietz, M. Bergmann, and K. Hartmann
  • Purification of histone lysine methyltransferase SMYD2 and co-crystallization with a target peptide from estrogen receptor [alpha] / Yuanyuan Jiang [and three others]
  • Gold nanoparticle-based Förester resonance energy transfer (FRET) analysis of estrogen receptor : DNA interaction / Xiaodi Su [and three others]
  • Analysis of interaction of estradiol with estrogen receptor by NMR spectroscopy / M.K. Thakur and V. Paramanik
  • Surface plasmon resonance study of cooperative interactions of estrogen receptor [alpha] and specificity protein 1 with composite DNA elements / Xiaodi Su and Hong Yan Song
  • Tips and tricks for successful application of statistical methods to biological data / Evelyn Schlenker
  • The synonymous Ala87 mutation of estrogen receptor alpha modifies transcriptional activation through both ERE and AP1 sites / Tamara Fernández-Calero, Gilles Flouriot, and Mónica Marín
  • Selective estrogen receptor modulators and the tissue-selective estrogen complex : analysis of cell type-specific effects using in vivo imaging of a reporter mouse model / Sara Della Torre and Paolo Ciana
  • Use of reporter genes to analyze estrogen response : the transgenic zebrafish model / Daniel A. Gorelick [and three others]
  • Comparison of the effects of the selective estrogen receptor modulators ospemifene, raloxifene, and tamoxifen on breast tissue in ex vivo culture / Natalija Eigeliene, Risto Erkkola, and Pirkko Härkönen
  • Estrogen receptor agonists and antagonists in the yeast estrogen bioassay / Si Wang and Toine F.H. Bovee
  • Silencing estrogen receptor-[alpha] with siRNA in the intact rodent brain / Ana C. Ribeiro [and three others]
  • Silencing estrogen receptor-[beta] with siRNA cultured cells / Ren-Jun Hsu and Jui-Ming Liu
  • Experimental procedures for demonstration of microRNA mediated enhancement of functional beuroprotective effects of estrogen receptor agonists / Mrinmay Chakrabarti and Swapan K. Ray
  • Expression profiles of estrogen-regulated microRNAs in breast cancer cells / Anne Katchy and Cecilia Williams
  • Estradiol-induced transcriptional regulation of long non-coding RNA, HOTAIR / Arunoday Bhan and Sunhrangsu S. Mandal
  • Detection and functional analysis of estrogen receptor [alpha] phosphorylated at serine 216 in mouse neutrophils / Sawako Shindo, Rick Moore, and Masahiko Negishi
  • Estrogen receptor-[alpha] knockout mice / Per Antonson, Patricia Humire, and Jan-Åke Gustafsson
  • Detection of endogenous selective estrogen receptor modulators such a 27-hydroxycholesterol / Erik R. Nelson
  • Phytoestrogens activate the estrogen receptor in HepG2 cells / Lynne A. Kelly
  • Detection of the phosphorylation of the estrogen receptor [alpha] as an outcome of GPR30 activation / Sara Clark [ and three others]
  • GPER mediates non-genomic effects of estrogen / Marco Pupo, Marcello Maggiolini, and Anna Maria Musti
  • GPER/GPR30 knockout mice : effects of GPER on metabolism / Geetanjali Sharma and Eric R. Prossnitz
  • Analysis of G-protein coupled receptor 30 (GPR30) on endothelial inflammation / Subhadeep Chakrabarti and Sandra T. Davidge
  • Atherosclerosis and vascular biologic responses to estrogens : histologic, immunohistochemical, biochemical, and molecular methods / Thomas C. Register, Susan E. Appt, and Thomas B. Clarkson
  • Assessing direct vascular actions of estrogens / Douglas S. Martin and Kathleen M. Eyster
  • Molecular design, synthesis, and evaluation of SNIPER (ER) that induces proteasomal degradation of ER[alpha] / Keiichiro Okuhira [ and six others]
  • Regulation of activation induced deaminase (AID) by estrogen / Siim Pauklin.
Description
Mode of access: World wide Web.
Notes
  • "Published In: United States, 13 November 2015 " --Distributor's website.
  • Includes bibliographical references and index.
Series Statement
Methods in molecular biology ; 1366
Methods in molecular biology (Clifton, N.J.) ; v. 1366
Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
  • Access in Virgo Classic
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    t| The estrogen receptors : an overview from different perspectives / r| Kathleen M. Eyster -- t| Competitive binding assay for the G-protein-coupled receptor 30 (GPR30) or G-protein-coupled estrogen receptor (GPER) / r| Thomas Thekkumkara, Russell Snyder, and Vardan T. Karamyan -- t| he use of real-time reverse transcription-PCR for assessing estrogen receptor and estrogen-responsive gene expression / r| Michelle L. Booze and Kathleen M. Eyster -- t| Bioinformatics analysis of estrogen-responsive genes / r| Adam E. Handel -- t| Electrophoretic mobility shift assay (EMSA) and supershift assay of cytochrome P450 2B6 in response to estrogen / r| Kwi Hye Koh and Hyunyoung Jeong -- t| Chromatin immunoprecipitation assay to identify genomic binding sites of regulatory factors / r| Meike Wagner [and five others] -- t| Chromatin immunoprecipitation with estrogen receptor 1 and the promoter of Greb1, in TM4 Sertoli cells / r| Jing Lin and Zhenmin Lei -- t| Chromatin immunoprecipitation-sequencing (ChIP-seq) for mapping of estrogen receptor-chromatin interactions in breast cancer / r| Kelly A. Holmes, Gordon D. Brown, and Jason S. Carroll -- t| RNA-Seq experiment and data analysis / r| Hanquan Liang and Erliang Zeng -- t| DNA microarray analysis of estrogen-responsive genes / r| Kathleen M. Eyster -- t| Shotgun proteomics analysis of estrogen effects in the uterus using two-dimensional liquid chromatography and tandem mass spectrometry / r| Eduardo A. Callegari -- t| Assessment of protein expression by proximity ligation assay in the nonhuman primate endometrium, placenta, and fetal adrenal in response to estrogen / r| Thomas W. Bonagura [and three others] -- t| Colocalization of estrogen receptors with the fluorescent tamoxifen derivative, FLTX1, analyzed by confocal microscopy / r| Araceli Morales [and four others] -- t| Live-cell imaging of the estrogen receptor by total internal reflection fluorescence microscopy / r| Kassandra Kisler and Reymundo Dominguez -- In situ hybridization of estrogen receptors [alpha] and [beta] and GPER in the human testis / r| Daniela Fietz, M. Bergmann, and K. Hartmann -- t| Purification of histone lysine methyltransferase SMYD2 and co-crystallization with a target peptide from estrogen receptor [alpha] / Yuanyuan Jiang [and three others] -- t| Gold nanoparticle-based Förester resonance energy transfer (FRET) analysis of estrogen receptor : DNA interaction / r| Xiaodi Su [and three others] -- Analysis of interaction of estradiol with estrogen receptor by NMR spectroscopy / r| M.K. Thakur and V. Paramanik -- t| Surface plasmon resonance study of cooperative interactions of estrogen receptor [alpha] and specificity protein 1 with composite DNA elements / r| Xiaodi Su and Hong Yan Song -- t| Tips and tricks for successful application of statistical methods to biological data / r| Evelyn Schlenker -- t| The synonymous Ala87 mutation of estrogen receptor alpha modifies transcriptional activation through both ERE and AP1 sites / r| Tamara Fernández-Calero, Gilles Flouriot, and Mónica Marín -- t| Selective estrogen receptor modulators and the tissue-selective estrogen complex : analysis of cell type-specific effects using in vivo imaging of a reporter mouse model / r| Sara Della Torre and Paolo Ciana -- t| Use of reporter genes to analyze estrogen response : the transgenic zebrafish model / r| Daniel A. Gorelick [and three others] -- t| Comparison of the effects of the selective estrogen receptor modulators ospemifene, raloxifene, and tamoxifen on breast tissue in ex vivo culture / r| Natalija Eigeliene, Risto Erkkola, and Pirkko Härkönen -- t| Estrogen receptor agonists and antagonists in the yeast estrogen bioassay / r| Si Wang and Toine F.H. Bovee -- Silencing estrogen receptor-[alpha] with siRNA in the intact rodent brain / r| Ana C. Ribeiro [and three others] -- t| Silencing estrogen receptor-[beta] with siRNA cultured cells / r| Ren-Jun Hsu and Jui-Ming Liu -- t| Experimental procedures for demonstration of microRNA mediated enhancement of functional beuroprotective effects of estrogen receptor agonists / r| Mrinmay Chakrabarti and Swapan K. Ray -- t| Expression profiles of estrogen-regulated microRNAs in breast cancer cells / r| Anne Katchy and Cecilia Williams -- t| Estradiol-induced transcriptional regulation of long non-coding RNA, HOTAIR / r| Arunoday Bhan and Sunhrangsu S. Mandal -- t| Detection and functional analysis of estrogen receptor [alpha] phosphorylated at serine 216 in mouse neutrophils / r| Sawako Shindo, Rick Moore, and Masahiko Negishi -- t| Estrogen receptor-[alpha] knockout mice / r| Per Antonson, Patricia Humire, and Jan-Åke Gustafsson -- t| Detection of endogenous selective estrogen receptor modulators such a 27-hydroxycholesterol / r| Erik R. Nelson -- t| Phytoestrogens activate the estrogen receptor in HepG2 cells / r| Lynne A. Kelly -- t| Detection of the phosphorylation of the estrogen receptor [alpha] as an outcome of GPR30 activation / r| Sara Clark [ and three others] -- t| GPER mediates non-genomic effects of estrogen / r| Marco Pupo, Marcello Maggiolini, and Anna Maria Musti -- t| GPER/GPR30 knockout mice : effects of GPER on metabolism / r| Geetanjali Sharma and Eric R. Prossnitz -- t| Analysis of G-protein coupled receptor 30 (GPR30) on endothelial inflammation / r| Subhadeep Chakrabarti and Sandra T. Davidge -- t| Atherosclerosis and vascular biologic responses to estrogens : histologic, immunohistochemical, biochemical, and molecular methods / r| Thomas C. Register, Susan E. Appt, and Thomas B. Clarkson -- t| Assessing direct vascular actions of estrogens / r| Douglas S. Martin and Kathleen M. Eyster -- t| Molecular design, synthesis, and evaluation of SNIPER (ER) that induces proteasomal degradation of ER[alpha] / r| Keiichiro Okuhira [ and six others] -- t| Regulation of activation induced deaminase (AID) by estrogen / r| Siim Pauklin.
    520
      
      
    a| This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen-- c| Source other than the Library of Congress.
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