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C. Elegans [electronic resource]: Methods and Applications

edited by David Biron, Department of Physics, The James Franck Institution, The Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA, Gal Haspel, Federated Department of Biological Sciences, New Jersey Institute of Technology and Rutgers University, Newark, NJ, USA
Format
EBook; Book; Online
Published
New York : Humana Press, [2015]
Edition
Second edition
Language
English
Variant Title
Caenorhabditis elegans
Series
Methods in Molecular Biology
Methods in Molecular Biology (Clifton, N.J.)
ISBN
9781493928415 (alk. paper), 1493928414 (alk. paper), 9781493928422 (ebook)
Contents
  • An overview of C. elegans biology / Kevin Strange
  • Comparative genomics in C. elegans, C. briggsae, and other Caenorhabditis species / Avril Coghlan, Jason E. Stajich, and Todd W. Harris
  • WormBase: methods for data mining and comparative genomics / Todd W. Harris and Lincoln D. Stein
  • C. elegans deletion mutant screening / Robert J. Barstead and Donald G. Moerman
  • Insertional mutagenesis in C. elegans using the drosophila transposon Mos1: a methods for the rapid identification of mutated genes / Jean-Louis Bessereau
  • Single nucleotide polymorphism mapping / M. Wayne Davis and Marc Hammarlund
  • Creation of transgenic lines using microparticle bombardment methods / Vida Praitis
  • Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNA / Lisa Timmons
  • Delivery methods for RNA interference in C. elegans / Lisa Timmons
  • Functional genomic approaches in C. elegans / Todd Lamitina
  • Assays for toxicity studies in C. elegans with Bt crystal proteins / Larry J. Bischof, Danielle L. Huffman, and Raffi V. Aroian
  • Fluorescent reporter methods / Harald Hutter
  • Electrophysiological analysis of neuronal and muscle function in C. elegans / Michael M. Francis and Andres Villu Maricq
  • Sperm and oocyte isolation methods for biochemical and proteomic analysis / Michael A. Miller
  • Preservation of C. elegans tissue via high-pressure freezing and freeze-substitution for ultrastructural analysis and immunocytochemistry / Robby M. Weimer
  • Intracellular pH measurements in vivo using green fluorescent protein variants / Keith Nehrke
  • Automated imaging of C. elegans behavior / Christopher J. Cronin, Zhaoyang Feng, and William R. Schafer
  • Intracellular Ca2+ imaging in C. elegans / Rex A. Kerr and William R. Schafer
  • In vitro culture of C. elegans somatic cells / Kevin Strange and Rebecca Morrison
  • Techniques for analysis, sorting, and dispensing of C. elegans on the COPAS flow-sorting system / Rock Pulak.
Description
Mode of access: World wide Web.
Series Statement
Methods in molecular biology, 1064-3745 ; 1327
Methods in molecular biology (Clifton, N.J.) ; v. 351
Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
  • Access in Virgo Classic
  • Staff View

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