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Notch Signaling [electronic resource]: Methods and Protocols

edited by Hugo J. Bellen, Department of Molecular and Human Genetics, Program in Developmental Biology, Department of Neuroscience, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA, Shinya Yamamoto, Department of Molecular and Human Gene...
Format
EBook; Book; Online
Published
New York : Humana Press, [2014]
Language
English
Uniform Title
Notch Signaling (Bellen)
Series
Methods in Molecular Biology
Springer Protocols
Methods in Molecular Biology (Clifton, N.J.)
Springer Protocols (Series)
ISBN
9781493911387 (alk. paper), 1493911384 (alk. paper), 9781493911394 (eBook), 1493911392 (eBook)
Contents
  • Introduction to notch signaling / Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen
  • Genetic screens to identify new notch pathway mutants in Drosophila / Nikolaos Giagtzoglou
  • Structure-function analysis of Drosophila notch using genomic tescue transgenes / Jessica Leonardi and Hamed Jafar-Nejad
  • Overview of genetic tools and techniques to study notch signaling in mice / Thomas Gridley and Andrew K. Groves
  • Immunohistochemical tools and techniques to visualize notch in Drosophila melanogaster / Emiliana Tognon and Thomas Vaccari
  • Antibody uptake assay and in vivo imaging to study intracellular trafficking of notch and delta in Drosophila / Lydie Couturier and François Schweisguth
  • Tracking trafficking of notch and its ligands in mammalian cells / Patricia Chastagner and Christel Brou
  • Visualizing notch signaling in vivo in Drosophila tissues / Benjamin E. Housden, Jinghua Li, and Sarah J. Bray
  • Monitoring notch activity in the mouse / Swananda Marathe and Lavinia Alberi
  • Notch signaling assays in Drosophila cultured cell lines / Jinghua Li, Benjamin E. Housden, and Sarah J. Bray
  • Monitoring notch activation in cultured mammalian cells : transcriptional reporter assays / Ma. Xenia G. Ilagan and Raphael Kopan
  • Monitoring notch activation in cultured mammalian cells : luciferase complementation imaging assays / Ma. Xenia G. Ilagan and Raphael Kopan
  • Visualization of notch signaling oscillation in cells and tissues / Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama
  • Proteomic analysis of the notch interactome / K. G. Guruharsha ... [et al.]
  • Bacterial expression and in vitro refolding of limited fragments of the notch receptor and its ligands / Pat Whiteman, Christina Redfield, and Penny A. Handford
  • Analyzing the posttranslational modification status of notch using mass spectrometry / Shinako Kakuda and Robert S. Haltiwanger
  • Assay to probe proteolytic processing of notch by [gamma]-secretase / Lutgarde Serneels, Ina Tesseur, and Bart De Strooper
  • Analyzing the nuclear complexes of notch signaling by electrophoretic mobility shift assay / Kelly L. Arnett and Stephen C. Blacklow
  • Identifying direct notch transcriptional targets using the GSI-washout assay / Will Bailis, Yumi Yashiro-Ohtani, and Warren S. Pear
  • Probing the epigenetic status at notch target genes / Robert Liefke and Tilman Borggrefe
  • Notch-ligand binding assays in Drosophila cells / Aiguo Xu and Kenneth D. Irvine
  • Modeling notch signaling : a practical tutorial / Pau Formosa-Jordan and David Sprinzak
  • Small molecules that inhibit notch signaling / Gerdien E. De Kloe and Bart De Strooper
  • Application and evaluation of anti-notch antibodies to modulate notch signaling / Wendy R. Gordon and Jon C. Aster
  • Application of anti-ligand antibodies to inhibit notch signaling / Jun-ichiro Koga and Masanori Aikawa.
Description
Mode of access: World wide Web.
Notes
Includes bibliographical references and index.
Series Statement
Methods in molecular biology, 1064-3745 ; 1187
Springer protocols, 1949-2448
Methods in molecular biology (Clifton, N.J.) ; v. 1187. 1064-3745
Springer protocols (Series) 1949-2448
Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
  • Access in Virgo Classic
  • Staff View

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    a| Notch signaling h| [electronic resource] : b| methods and protocols / c| edited by Hugo J. Bellen, Department of Molecular and Human Genetics, Program in Developmental Biology, Department of Neuroscience, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA, Shinya Yamamoto, Department of Molecular and Human Genetics, Program in Developmental Biology, Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, TX, USA.
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    t| Introduction to notch signaling / r| Shinya Yamamoto, Karen L. Schulze, and Hugo J. Bellen -- t| Genetic screens to identify new notch pathway mutants in Drosophila / r| Nikolaos Giagtzoglou -- t| Structure-function analysis of Drosophila notch using genomic tescue transgenes / r| Jessica Leonardi and Hamed Jafar-Nejad -- t| Overview of genetic tools and techniques to study notch signaling in mice / r| Thomas Gridley and Andrew K. Groves -- t| Immunohistochemical tools and techniques to visualize notch in Drosophila melanogaster / r| Emiliana Tognon and Thomas Vaccari -- t| Antibody uptake assay and in vivo imaging to study intracellular trafficking of notch and delta in Drosophila / r| Lydie Couturier and François Schweisguth -- t| Tracking trafficking of notch and its ligands in mammalian cells / r| Patricia Chastagner and Christel Brou -- t| Visualizing notch signaling in vivo in Drosophila tissues / r| Benjamin E. Housden, Jinghua Li, and Sarah J. Bray -- t| Monitoring notch activity in the mouse / r| Swananda Marathe and Lavinia Alberi -- t| Notch signaling assays in Drosophila cultured cell lines / r| Jinghua Li, Benjamin E. Housden, and Sarah J. Bray -- t| Monitoring notch activation in cultured mammalian cells : transcriptional reporter assays / r| Ma. Xenia G. Ilagan and Raphael Kopan -- t| Monitoring notch activation in cultured mammalian cells : luciferase complementation imaging assays / r| Ma. Xenia G. Ilagan and Raphael Kopan -- t| Visualization of notch signaling oscillation in cells and tissues / r| Hiromi Shimojo, Yukiko Harima, and Ryoichiro Kageyama -- t| Proteomic analysis of the notch interactome / r| K. G. Guruharsha ... [et al.] -- t| Bacterial expression and in vitro refolding of limited fragments of the notch receptor and its ligands / r| Pat Whiteman, Christina Redfield, and Penny A. Handford -- t| Analyzing the posttranslational modification status of notch using mass spectrometry / r| Shinako Kakuda and Robert S. Haltiwanger -- t| Assay to probe proteolytic processing of notch by [gamma]-secretase / r| Lutgarde Serneels, Ina Tesseur, and Bart De Strooper -- t| Analyzing the nuclear complexes of notch signaling by electrophoretic mobility shift assay / r| Kelly L. Arnett and Stephen C. Blacklow -- t| Identifying direct notch transcriptional targets using the GSI-washout assay / r| Will Bailis, Yumi Yashiro-Ohtani, and Warren S. Pear -- t| Probing the epigenetic status at notch target genes / r| Robert Liefke and Tilman Borggrefe -- t| Notch-ligand binding assays in Drosophila cells / r| Aiguo Xu and Kenneth D. Irvine -- t| Modeling notch signaling : a practical tutorial / r| Pau Formosa-Jordan and David Sprinzak -- t| Small molecules that inhibit notch signaling / r| Gerdien E. De Kloe and Bart De Strooper -- t| Application and evaluation of anti-notch antibodies to modulate notch signaling / r| Wendy R. Gordon and Jon C. Aster -- t| Application of anti-ligand antibodies to inhibit notch signaling / r| Jun-ichiro Koga and Masanori Aikawa.
    538
      
      
    a| Mode of access: World wide Web.
    650
      
    0
    a| Notch genes v| Laboratory manuals.
    650
      
    0
    a| Notch proteins v| Laboratory manuals.
    650
    1
    2
    a| Receptors, Notch v| Laboratory Manuals.
    650
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    2
    a| Signal Transduction v| Laboratory Manuals.
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    a| Electronic books.
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    a| Methods in molecular biology (Clifton, N.J.) ; v| v. 1187. x| 1064-3745
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    a| Springer protocols (Series) x| 1949-2448
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