Item Details

Ras Signaling [electronic resource]: Methods and Protocols

edited by Lorenza Trabalzini, Department of Biotechnology, Chemistry, and Pharmacy, University of Siena, Siena, Italy, Saverio Francesco Retta, Department of Clinical and Biological Sciences, University of Torino, Torino, Italy
Format
EBook; Book; Online
Published
New York : Humana Press, [2014]
Language
English
Series
Methods in Molecular Biology
Springer Protocols
Methods in Molecular Biology (Clifton, N.J.)
Springer Protocols (Series)
ISBN
9781627037907 (alk. paper), 162703790X (alk. paper), 9781627037914 (eBook)
Contents
  • The Ras superfamily of small GTPases : the unlocked secrets / Luca Goitre [and three others]
  • A novel method for the production of fully modified K-Ras 4B / Tanmay S. Chavan [and four others]
  • Metabolic labeling of Ras with tritiated palmitate to monitor palmitoylation and depalmitoylation / Frederick D. Tsai [and three others]
  • Ubiquitination of Rac1 by inhibitors of apoptosis (IAPs) / Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam
  • Ras GTPases are both regulators and effectors of redox agents / Elisa Ferro [and four others]
  • Biophysical and proteomic characterization strategies for cysteine modifications in Ras GTPases / G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell
  • Use of the yeast two-hybrid technology to isolate molecular interactions of Ras GTPases / Elisa Ferro, Eva Baldini, and Lorenza Trabalzini
  • Screening for MAPK modulators using an In-Cell Western assay / Simon Schnaiter [and seven others]
  • Behavioral methods for the study of the Ras-ERK pathway in memory formation and consolidation : passive avoidance and novel object recognition tests / Raffaele d'Isa, Riccardo Brambilla, and Stefania Fasano
  • Functional phosphoproteomics of oncogenic KRAS signaling / Putty-Reddy Sudhir and Jeou-Yuan Chen
  • Pull-down assay for analysis of integrin-mediated activation of Rap proteins in adherent platelets / Gianni Francesco Guidetti and Mauro Torti
  • Combined pulldown and time-lapse microscopy studies for determining the role of Rap1 in the crosstalk between integrins and cadherins / Luca Goitre and Saverio Francesco Retta
  • Fluorescence microscopy study of Rap1 subcellular localization / Luca Goitre, Valentina Cutano, and Saverio Francesco Retta
  • An in vitro system to evaluate the scaffold function of the RalA effector protein RalBP1 / David F. Kashatus
  • Analysis of the Rit subfamily GTPase-mediated signaling and neuronal differentiation and survival / Geng-Xian Shi and Catherine N. Kaminski
  • Immunofluorescence methods in studies of the GTPase Ran and its effectors in interphase and in mitotic cells / Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia
  • High-resolution scanning electron microscopy for the imaging of nuclear pore complexes and Ran-mediated transport / Lihi Shaulov, Boris Fichtman, and Amnon Harel
  • Effector recruitment method to study spatially regulated activation of Ras and Rho GTPases / Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox
  • Real-time visualization and quantification of native Ras activation in single living cells / Christoph Biskup and Ignacio Rubio
  • Nanoclustering and heterogeneous membrane diffusion of Ras studied by FRAP and RICS analysis / Camilo Guzmán, Maja Šolman, and Daniel Abankwa
  • Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay / Audrey Colomba and Anne J. Ridley
  • Analysis of Rho GTPase-induced localization of nanoscale adhesions using fluorescence nanoscopy / Annica K.B. Gad [and three others]
  • Yeast as a model for Ras signalling / Renata Tisi, Fiorella Belotti, and Enzo Martegani
  • Methods to study the Ras2 protein activation state and the subcellular localization of Ras-GTP in Saccharomyces cerevisiae / Sonia Colombo and Enzo Martegani
  • Ras proteins signaling in the early metazoan Dictyostelium discoideum / Enrico Bracco and Barbara Pergolizzi.
Description
Mode of access: World wide Web.
Notes
Includes bibliographical references and index.
Series Statement
Methods in molecular biology, 1064-3745 ; 1120
Springer protocols, 1949-2448
Methods in molecular biology (Clifton, N.J.) ; v. 1120, 1064-3745
Springer protocols (Series) 1949-2448
Logo for Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
  • Access in Virgo Classic

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    t| The Ras superfamily of small GTPases : the unlocked secrets / r| Luca Goitre [and three others] -- t| A novel method for the production of fully modified K-Ras 4B / r| Tanmay S. Chavan [and four others] -- t| Metabolic labeling of Ras with tritiated palmitate to monitor palmitoylation and depalmitoylation / r| Frederick D. Tsai [and three others] -- t| Ubiquitination of Rac1 by inhibitors of apoptosis (IAPs) / r| Tripat Kaur Oberoi-Khanuja and Krishnaraj Rajalingam -- t| Ras GTPases are both regulators and effectors of redox agents / r| Elisa Ferro [and four others] -- t| Biophysical and proteomic characterization strategies for cysteine modifications in Ras GTPases / r| G. Aaron Hobbs, Harsha P. Gunawardena, and Sharon L. Campbell -- t| Use of the yeast two-hybrid technology to isolate molecular interactions of Ras GTPases / r| Elisa Ferro, Eva Baldini, and Lorenza Trabalzini -- t| Screening for MAPK modulators using an In-Cell Western assay / r| Simon Schnaiter [and seven others] -- t| Behavioral methods for the study of the Ras-ERK pathway in memory formation and consolidation : passive avoidance and novel object recognition tests / r| Raffaele d'Isa, Riccardo Brambilla, and Stefania Fasano -- t| Functional phosphoproteomics of oncogenic KRAS signaling / r| Putty-Reddy Sudhir and Jeou-Yuan Chen -- t| Pull-down assay for analysis of integrin-mediated activation of Rap proteins in adherent platelets / r| Gianni Francesco Guidetti and Mauro Torti -- t| Combined pulldown and time-lapse microscopy studies for determining the role of Rap1 in the crosstalk between integrins and cadherins / r| Luca Goitre and Saverio Francesco Retta -- t| Fluorescence microscopy study of Rap1 subcellular localization / r| Luca Goitre, Valentina Cutano, and Saverio Francesco Retta -- t| An in vitro system to evaluate the scaffold function of the RalA effector protein RalBP1 / r| David F. Kashatus -- t| Analysis of the Rit subfamily GTPase-mediated signaling and neuronal differentiation and survival / r| Geng-Xian Shi and Catherine N. Kaminski -- t| Immunofluorescence methods in studies of the GTPase Ran and its effectors in interphase and in mitotic cells / r| Giulia Guarguaglini, Valeria de Turris, and Patrizia Lavia -- t| High-resolution scanning electron microscopy for the imaging of nuclear pore complexes and Ran-mediated transport / r| Lihi Shaulov, Boris Fichtman, and Amnon Harel -- t| Effector recruitment method to study spatially regulated activation of Ras and Rho GTPases / r| Lauren P. Huff, Molly J. DeCristo, and Adrienne D. Cox -- t| Real-time visualization and quantification of native Ras activation in single living cells / r| Christoph Biskup and Ignacio Rubio -- t| Nanoclustering and heterogeneous membrane diffusion of Ras studied by FRAP and RICS analysis / r| Camilo Guzmán, Maja Šolman, and Daniel Abankwa -- t| Analyzing the roles of Rho GTPases in cancer cell migration with a live cell imaging 3D-morphology-based assay / r| Audrey Colomba and Anne J. Ridley -- t| Analysis of Rho GTPase-induced localization of nanoscale adhesions using fluorescence nanoscopy / r| Annica K.B. Gad [and three others] -- t| Yeast as a model for Ras signalling / r| Renata Tisi, Fiorella Belotti, and Enzo Martegani -- t| Methods to study the Ras2 protein activation state and the subcellular localization of Ras-GTP in Saccharomyces cerevisiae / r| Sonia Colombo and Enzo Martegani -- t| Ras proteins signaling in the early metazoan Dictyostelium discoideum / r| Enrico Bracco and Barbara Pergolizzi.
    538
      
      
    a| Mode of access: World wide Web.
    650
      
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    a| Ras oncogenes v| Laboratory manuals.
    650
      
    0
    a| Ras proteins v| Laboratory manuals.
    650
      
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    a| Cellular signal transduction v| Laboratory manuals.
    650
      
    0
    a| Guanosine triphosphatase v| Laboratory manuals.
    650
      
    0
    a| Guanosine triphosphatase genes v| Laboratory manuals.
    650
      
    0
    a| GTPase-activating protein v| Laboratory manuals.
    650
      
    0
    a| Cell transformation v| Laboratory manuals.
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    a| ras Proteins v| Laboratory Manuals.
    650
      
    2
    a| Signal Transduction v| Laboratory Manuals.
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