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Quantitative Real-Time PCR [electronic resource]: Methods and Protocols

edited by Roberto Biassoni, Laboratorio Medicina Molecolare, Dipartimento Medicina Traslazionale, Instituto Giannina Gaslini, Genova, Italy, Alessandro Raso, Laboratorio U.O.C. Neurochirurgia, Instituto Giannina Gaslini, Genova, Italy
Format
EBook; Book; Online
Published
New York : Humana Press, [2014]
Language
English
Series
Methods in Molecular Biology
Springer Protocols
Methods in Molecular Biology (Clifton, N.J.)
Springer Protocols (Series)
ISBN
9781493907328 (acid-free paper), 1493907328 (acid-free paper), 9781493907335 (ebook)
Summary
Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ? Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation. --
Contents
  • Twenty years of qPCR: A mature technology?
  • Minimum information necessary for quantitative real-time PCR experiments
  • Selection of reliable reference genes for RT-qPCR analysis
  • Introduction to digital PCR
  • mRNA and microRNA purity and integrity: The key to success in expression profiling
  • Mediator probe PCR: Detection of real-time PCR by label-free probes and a universal fluorogenic reporter
  • Absolute quantification of viral DNA: The quest for perfection
  • A multiplex real-time PCR-platform integrated into automated extraction method for the rapid detection and measurement of oncogenic HPV type-specific viral DNA load from cervical samples
  • Real-time PCR detection of mycoplasma pneumoniae in the diagnosis of community-acquired pneumonia
  • A sensible technique to detect mollicutes impurities in human cells cultured in GMP condition
  • Real-time quantification assay to monitor BCR-ABL1 transcripts in chronic myeloid leukemia
  • A reliable assay for rapidly defining transplacental metastasis using quantitative PCR
  • Circulating cell-free DNA in cancer
  • Gene expression analysis by qPCR in clinical kidney transplantation
  • Posttranscriptional regulatory networks: From expression profiling to integrative analysis of mRNA and microRNA data
  • Clinical applications using digital PCR
  • Developing noninvasive diagnosis for single-gene disorders: the role of digital PCR.
Description
Mode of access: World wide Web.
Notes
Includes bibliographical references and index.
Series Statement
Methods in molecular biology, 1064-3745 ; 1160
Methods in molecular biology (Clifton, N.J.) ; v. 1160. 1064-3745
Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
  • Access in Virgo Classic
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    t| Twenty years of qPCR: A mature technology? -- t| Minimum information necessary for quantitative real-time PCR experiments -- t| Selection of reliable reference genes for RT-qPCR analysis -- t| Introduction to digital PCR -- t| mRNA and microRNA purity and integrity: The key to success in expression profiling -- t| Mediator probe PCR: Detection of real-time PCR by label-free probes and a universal fluorogenic reporter -- t| Absolute quantification of viral DNA: The quest for perfection -- t| A multiplex real-time PCR-platform integrated into automated extraction method for the rapid detection and measurement of oncogenic HPV type-specific viral DNA load from cervical samples -- t| Real-time PCR detection of mycoplasma pneumoniae in the diagnosis of community-acquired pneumonia -- t| A sensible technique to detect mollicutes impurities in human cells cultured in GMP condition -- t| Real-time quantification assay to monitor BCR-ABL1 transcripts in chronic myeloid leukemia -- t| A reliable assay for rapidly defining transplacental metastasis using quantitative PCR -- t| Circulating cell-free DNA in cancer -- t| Gene expression analysis by qPCR in clinical kidney transplantation -- t| Posttranscriptional regulatory networks: From expression profiling to integrative analysis of mRNA and microRNA data -- t| Clinical applications using digital PCR -- t| Developing noninvasive diagnosis for single-gene disorders: the role of digital PCR.
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