Item Details

Mitosis [electronic resource]: Methods and Protocols

edited by David J. Sharp, Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY, USA
Format
EBook; Book; Online
Published
New York : Humana Press, [2014]
Language
English
Series
Methods in Molecular Biology
Springer Protocols
Methods in Molecular Biology (Clifton, N.J.)
Springer Protocols (Series)
ISBN
9781493903283 (hardback : alk. paper), 1493903284 (hardback : alk. paper), 9781493903290 (eBook)
Summary
Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods pertaining to distinct aspects of mitosis research. Part I presents approaches for visualizing and analyzing the dynamic behaviors of the spindle apparatus, the microtubule based machine that drives chromosome segregation. Part II focuses more generally on methods for studying and manipulating the microtubule cytoskeleton in cells and complex cell free extracts. Part III provides state of the art biophysical and high resolution microscopy approaches for assessing complex interactions between microtubules and microtubule-associated proteins in isolation as well as microtubule structure in cells. Part IV provides methods for studying the effects of cell shape on cell division, and methods for quantifying aneuploidy (aberrant chromosome number) which frequently results from mitotic defects and has been linked to human maladies ranging from birth defects to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitosis: Methods and Protocols seeks to provide diverse methods and new techniques to address new or old questions related to the mechanisms of mitosis.--
Contents
  • Analysis of Mitotic Protein Dynamics and Function in Drosophila Embryos by Live Cell Imaging and Quantitative Modeling
  • Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells
  • Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Micro copy Images of C. elegans Embryos
  • Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells
  • Image-Based Computational Tracking and Analysis of Spindle Protein Dynamics
  • The Use of Cultured Drosophila Cells for Studying the Microtubule Cytoskeleton
  • Measuring Microtubule Growth and Gliding in C. elegans Embryos
  • XenopusEgg Extracts as a Simplified Model System for Structure Function Studies of Dynein Regulators
  • Covalent Immobilization of Microtubules on Glass Surfaces for Molecular Motor Force Measurements and Other Single-Molecule Assays
  • An Improved Optical Tweezers Assay for Measuring the Force Generation of Single Kinesin Molecules
  • Seeded Microtubule Growth for Cryo-electron Microscopy of End Binding Proteins
  • The Segmentation of Microtubules in Electron Tomograms using Amira
  • Manipulating Cell Shape by Placing Cells into Micro-fabricated Chambers
  • Four color FISH for the Detection of Low Levels Aneuploidy in Interphase Cells.
Description
Mode of access: World wide Web.
Notes
Includes bibliographical references and index.
Series Statement
Methods in molecular biology, 1064-3745 ; 1136
Methods in molecular biology (Clifton, N.J.) ; v. 1136. 1064-3745
Logo for Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
  • Access in Virgo Classic

  • LEADER 04910nam a22005177a 4500
    001 u6403780
    003 SIRSI
    005 20140918132457.0
    006 m d
    007 cr n
    008 140129t20142014nyua sb 001 0 eng d
    010
      
      
    a| 2014931984
    015
      
      
    a| GBB421320 2| bnb
    016
    7
      
    a| 101634365 2| DNLM
    016
    7
      
    a| 016635866 2| Uk
    020
      
      
    a| 9781493903283 (hardback : alk. paper)
    020
      
      
    a| 1493903284 (hardback : alk. paper)
    020
      
      
    z| 9781493903290 (eBook)
    035
      
      
    a| (WaSeSS)ssj0001287023
    040
      
      
    a| NLM b| eng c| NLM d| UKMGB d| YDXCP d| DEBBG d| N15 d| OHX d| IDL d| NLGGC d| NUI d| STF d| OCLCO d| DLC d| WaSeSS
    042
      
      
    a| nlmcopyc a| lccopycat
    050
    0
    0
    a| QH605.2 b| .M565 2014
    060
    0
    0
    a| W1 b| ME9616J v.1136 2014
    060
    1
    0
    a| QU 25
    072
      
    7
    a| QH 2| lcco
    082
    0
    4
    a| 571.844 2| 23
    084
      
      
    a| WG 2100 2| rvk
    245
    0
    0
    a| Mitosis h| [electronic resource] : b| methods and protocols / c| edited by David J. Sharp, Department of Physiology and Biophysics, Albert Einstein College of Medicine, Bronx, NY, USA.
    260
      
      
    a| New York : b| Humana Press, c| [2014]
    490
    1
      
    a| Methods in molecular biology, x| 1064-3745 ; v| 1136
    490
    1
      
    a| Springer protocols
    504
      
      
    a| Includes bibliographical references and index.
    505
    0
      
    a| Analysis of Mitotic Protein Dynamics and Function in Drosophila Embryos by Live Cell Imaging and Quantitative Modeling -- Rapid Measurement of Mitotic Spindle Orientation in Cultured Mammalian Cells -- Automated Segmentation of the First Mitotic Spindle in Differential Interference Contrast Micro copy Images of C. elegans Embryos -- Imaging the Mitotic Spindle by Spinning Disk Microscopy in Tobacco Suspension Cultured Cells -- Image-Based Computational Tracking and Analysis of Spindle Protein Dynamics -- The Use of Cultured Drosophila Cells for Studying the Microtubule Cytoskeleton -- Measuring Microtubule Growth and Gliding in C. elegans Embryos -- XenopusEgg Extracts as a Simplified Model System for Structure Function Studies of Dynein Regulators -- Covalent Immobilization of Microtubules on Glass Surfaces for Molecular Motor Force Measurements and Other Single-Molecule Assays -- An Improved Optical Tweezers Assay for Measuring the Force Generation of Single Kinesin Molecules -- Seeded Microtubule Growth for Cryo-electron Microscopy of End Binding Proteins -- The Segmentation of Microtubules in Electron Tomograms using Amira -- Manipulating Cell Shape by Placing Cells into Micro-fabricated Chambers -- Four color FISH for the Detection of Low Levels Aneuploidy in Interphase Cells.
    520
      
      
    a| Mitosis: Methods and Protocols provides state-of-the-art overviews on the most important approaches currently used in mitosis research spanning from the analysis of single molecules in isolation to their utilization within the complex environment of the cell. The volume is divided into four parts, each focused on methods pertaining to distinct aspects of mitosis research. Part I presents approaches for visualizing and analyzing the dynamic behaviors of the spindle apparatus, the microtubule based machine that drives chromosome segregation. Part II focuses more generally on methods for studying and manipulating the microtubule cytoskeleton in cells and complex cell free extracts. Part III provides state of the art biophysical and high resolution microscopy approaches for assessing complex interactions between microtubules and microtubule-associated proteins in isolation as well as microtubule structure in cells. Part IV provides methods for studying the effects of cell shape on cell division, and methods for quantifying aneuploidy (aberrant chromosome number) which frequently results from mitotic defects and has been linked to human maladies ranging from birth defects to cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitosis: Methods and Protocols seeks to provide diverse methods and new techniques to address new or old questions related to the mechanisms of mitosis.-- c| Source other than Library of Congress.
    538
      
      
    a| Mode of access: World wide Web.
    650
      
    0
    a| Mitosis.
    650
      
    0
    a| Mitosis x| Research x| Methodology.
    650
    1
    2
    a| Mitosis x| physiology v| Laboratory Manuals.
    655
      
    0
    a| Electronic books.
    700
    1
      
    a| Sharp, David, d| 1968-
    710
    2
      
    a| Springer Protocols
    830
      
    0
    a| Methods in molecular biology (Clifton, N.J.) ; v| v. 1136. x| 1064-3745
    830
      
    0
    a| Springer protocols (Series)
    856
    4
    0
    u| http://RE5QY4SB7X.search.serialssolutions.com/?V=1.0&L=RE5QY4SB7X&S=JCs&C=TC0001287023&T=marc
    596
      
      
    a| 1
    999
      
      
    a| XX(6403780.1) w| WEB i| 6403780-1001 l| INTERNET m| UVA-LIB t| INTERNET

Availability

Google Preview

Read Online