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Staphylococcus Epidermidis [electronic resource]: Methods and Protocols

edited by Paul D. Fey, Department of Pathology and Microbiology, Center for Staphylococcal Research, University of Nebraska Medical Center, Omaha, NE, USA
Format
EBook; Book; Online
Published
New York : Humana Press, [2014]
Language
English
Series
Methods in Molecular Biology
Springer Protocols
Methods in Molecular Biology (Clifton, N.J.)
Springer Protocols (Series)
ISBN
1627037357 (alk. paper), 9781627037358 (alk. paper), 9781627037365 (eBook)
Summary
Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. --
Contents
  • Clinical characteristics of infections in humans due to Staphylococcus epidermidis / Mark E. Rupp
  • Staphylococcus epidermidis pathogenesis / Michael Otto
  • Identification of Staphylococcus epidermidis in the clinical microbiology laboratory by molecular methods / Amity L. Roberts
  • Pulsed field gel electrophoresis of Staphylococcus epidermidis / Richard V. Goering and Paul D. Fey
  • Multilocus sequence typing of Staphylococcus epidermidis / Jonathan C. Thomas and D. Ashley Robinson
  • Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis / Greg A. Somerville and Robert Powers
  • The isolation and analysis of phenol-soluble modulins of Staphylococcus epidermidis / Hwang-Soo Joo and Michael Otto
  • Genetic manipulation of staphylococci / Jeffrey L. Bose
  • Isolation of chromosomal and plasmid DNA from Staphylococcus epidermidis / Jill K. Lindgren
  • Isolation of Staphylococus sp. RNA / Tess Eidem
  • Use of electroporation and conjugative mobilization for genetic manipulation of Staphylococcus epidermidis / Katherine L. Maliszewski and Austin S. Nuxoll
  • Methods to generate a sequence-defined transposon mutant library in Staphylococcus epidermidis strain 1457 / Todd J. Widhelm [and four others]
  • Examination of Staphylococcus epidermidis biofilms using flow-cell technology / Derek E. Moormeier and Kenneth W. Bayles
  • Rapid quantitative and qualitative analysis of biofilm production by Staphylococcus epidermidis under static growth conditions / Elaine M. Waters [and five others]
  • Bacteriophage transduction in Staphylococcus epidermidis / Michael E. Olson and Alexander R. Horswill
  • Mouse model of post-arthroplasty Staphylococcus epidermidis joint infection / Tyler D. Scherr [and five others]
  • A mouse model of Staphylococcus catheter-associated biofilm infection / Cortney E. Heim, Mark L. Hanke, and Tammy Kielian
  • Generation of a central nervous system catheter-associated infection in mice with Staphylococcus epidermidis / Jessica N. Snowden
  • Rat jugular catheter model of biofilm-mediated infection / Carolyn R. Schaeffer, Keith M. Woods, and G. Matthew Longo.
Description
Mode of access: World wide Web.
Notes
Includes bibliographical references and index.
Series Statement
Methods in molecular biology, 1064-3745 ; 1106
Springer protocols, 1949-2448
Methods in molecular biology (Clifton, N.J.) ; 1106. 1064-3745
Springer protocols (Series) 1949-2448
Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
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    a| Clinical characteristics of infections in humans due to Staphylococcus epidermidis / Mark E. Rupp -- Staphylococcus epidermidis pathogenesis / Michael Otto -- Identification of Staphylococcus epidermidis in the clinical microbiology laboratory by molecular methods / Amity L. Roberts -- Pulsed field gel electrophoresis of Staphylococcus epidermidis / Richard V. Goering and Paul D. Fey -- Multilocus sequence typing of Staphylococcus epidermidis / Jonathan C. Thomas and D. Ashley Robinson -- Growth and preparation of Staphylococcus epidermidis for NMR metabolomic analysis / Greg A. Somerville and Robert Powers -- The isolation and analysis of phenol-soluble modulins of Staphylococcus epidermidis / Hwang-Soo Joo and Michael Otto -- Genetic manipulation of staphylococci / Jeffrey L. Bose -- Isolation of chromosomal and plasmid DNA from Staphylococcus epidermidis / Jill K. Lindgren -- Isolation of Staphylococus sp. RNA / Tess Eidem -- Use of electroporation and conjugative mobilization for genetic manipulation of Staphylococcus epidermidis / Katherine L. Maliszewski and Austin S. Nuxoll -- Methods to generate a sequence-defined transposon mutant library in Staphylococcus epidermidis strain 1457 / Todd J. Widhelm [and four others] -- Examination of Staphylococcus epidermidis biofilms using flow-cell technology / Derek E. Moormeier and Kenneth W. Bayles -- Rapid quantitative and qualitative analysis of biofilm production by Staphylococcus epidermidis under static growth conditions / Elaine M. Waters [and five others] -- Bacteriophage transduction in Staphylococcus epidermidis / Michael E. Olson and Alexander R. Horswill -- Mouse model of post-arthroplasty Staphylococcus epidermidis joint infection / Tyler D. Scherr [and five others] -- A mouse model of Staphylococcus catheter-associated biofilm infection / Cortney E. Heim, Mark L. Hanke, and Tammy Kielian -- Generation of a central nervous system catheter-associated infection in mice with Staphylococcus epidermidis / Jessica N. Snowden -- Rat jugular catheter model of biofilm-mediated infection / Carolyn R. Schaeffer, Keith M. Woods, and G. Matthew Longo.
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    a| Due to the highly collaborative nature of investigators working in the field, we have rapidly advanced our understanding of Staphylococcus epidermidis and other staphylococci in the last two decades. The chapters in Staphylococcus Epidermidis: Methods and Protocols are designed to give the new investigator a series of tools so they can ask novel and exciting questions related to the biology of this opportunistic pathogen, as many exciting and unexplored questions such as defining the interaction of S. epidermidis and other normal flora remain to be discovered. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. -- c| Source other than Library of Congress.
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