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Neural Progenitor Cells [electronic resource]: Methods and Protocols

edited by Brent A. Reynolds, Department of Neurosurgery, University of Florida, Gainesville, FL, USA, Loic P. Deleyrolle, Department of Neurosurgery, University of Florida, Gainesville, FL, USA
Format
EBook; Book; Online
Published
New York : Humana Press, [2013]
Language
English
Series
Methods in molecular biology
Springer protocols
Methods in molecular biology (Clifton, N.J.)
Springer protocols (Series)
ISBN
9781627035736 (alk. paper), 1627035737 (alk. paper), 9781627035743 (eBook)
Summary
"The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing "no new neuron" doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls." --
Contents
  • 1. Culturing fetal precursor cells using free floating serum-free conditions / Andrew Chojnacki and Samuel Weiss
  • 2. In vitro expansion of fetal neural progenitors as adherent cell lines / Steven M. Pollard
  • 3. Isolate and culture precursor cells from the adult periventricular area / Chiara Cavazzin, Margherita Neri and Angela Gritti
  • 4. Culturing and expansion of precursor cells from the adult hippocampus / Dhanisha J. Jhaveri, Boris W. Prosper and Perry F. Bartlett
  • 5. Isolate and culture neural stem cells from the mouse adult spinal cord / Jean-Philippe Hugnot
  • 6. Culturing and expansion of "clinical grade" precursors cells from the fetal human central nervous system / Maurizio Gelati, Daniela Profico, Massimo Projetti-Pensi, Gianmarco Muzi, Giada Sgaraavizzi and Anagel Luigi Vescovi
  • 7. Isolating and culturing of precursor cells from the adult human brain / Florian A. Siebzehnrubl and Dennis A. Steindler
  • 8. Isolation and culture of precursor cells from the adult human spinal cord / Luc Bauchet, Nicolas Lonjon, Florence Vachiery-Lahaye, Alain Boularan, Alain Privat and Jean-Philippe Hugnot
  • 9. Isolation and enrichment of defined neural cell populations from heterogeneous neural stem cell progeny / Hassan Azari
  • 10. Isolation of adult stem cells from the human olfactory mucosa / François Féron, Chris Perry, Stéphane D. Girard and Alan Mackay-Sim
  • 11. Enumerating stem cell frequency: neural colony forming cell assay / Sharon A. Louis and Carmen K.H. Mak
  • 12. Flow cytometry of neural cells / Geoffrey W. Osborne
  • 13. Neonatal transplant in hypoxic injury / Tong Zheng and Michael D. Weiss
  • 14. Isolation and purification of self-renewable human neural stem cells for cell therapy in experimental model of Ischemic stroke / Ricardo L. Azevedo-Pereira and Marcel M. Daadi
  • 15.Transplantation of fetal midbrain dopamine progenitors into a rodent model of Parkinson's disease / Lachlan H. Thompson and Clare L. Parish
  • 16. Distribution of neural precursor cells in the adult mouse brain / Daniel G. Blackmore and Rodney L. Rietze
  • 17. Identifying neural progenitor cells in the adult human brain / Thomas I.H. Park, Henry J. Waldvogel, Johananna M. Montgomery, Edward W.Mee, Peter S. Bergin, Richard L.M. Faull, Mike Dragunow and Maurice A. Curtis
  • 18. Clinical trials for the treatment of spinal cord injury: not so simple / Alan Mackay-Sim and François Féron.
Description
Mode of access: World wide Web.
Notes
Includes bibliographical references and index.
Series Statement
Methods in molecular biology, 1064-3745 ; 1059
Springer protocols, 1949-2448
Methods in molecular biology (Clifton, N.J.) ; 1059
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