Item Details

Chromatin and Chromatin Remodeling Enzymes: Part C

edited by C. David Allis, Carl Wu
Format
Book
Published
San Diego : Elsevier/ Academic Press, c2004.
Language
English
Series
Methods in Enzymology
ISBN
012182781X
Contents
  • 1. Functional analyses of chromatin modifications in yeast
  • 2. Genetic analysis of chromatin remodeling using budding yeast as a model
  • 3. Introduction to Trx-G and Pc-G genes
  • 4. Genetic and cytological analysis of drosophila chromatin- remodeling factors
  • 5. Genetic analysis if H1 linker histone subtypes and their functions in mice
  • 6. The use of mass spectrometry for the analysis of histone modifications
  • 7. Histone modification patterns during gene activation
  • 8. Identification and analysis of native HAT complexes
  • 9. Histone deacetylases: purification of the enzymes, substrates, and assay conditions
  • 10. Measurement of mammalian histone deacetylase activity
  • 11. Analysis of histone phosphorylation: coupling intracellular signaling to chromatin remodeling
  • 12. Purification of histone methyltransferases from HeLa cells
  • 13. Global proteomic analysis of s. cerevisiae (GPS) to identify proteins required for histone modifications
  • 14. Purification of Sir2 proteins from yeast
  • 15. Purification and biochemical properties of the drosophila TAC1 complex
  • 16. Isolation and characterization of CHRASCH, a polycomb-containing silencing complex
  • 17. Biochemical analysis of mammalian polycomb group protein complexes and the identification of genetic elements that block polycomb-mediated gene repression
  • 18. Purification and functional analysis of the mammalian SWI/SNF-family of chromatin-remodeling complexes
  • 19. Isolation and assay of the RSC chromatin-remodeling complex from saccharomyces cerevisiae
  • 20. DNA translocation and nucleosome remodeling assays by the RSC chromatin remodeling complex
  • 21. A Necleosome sliding assay for chromatin remodeling factors
  • 22. Methods for analysis of nucleosome sliding by drosophila NURF
  • 23. Methods for preparation and assays for xenopus ISWI complexes
  • 24. Functional analysis or ISWI complexes in mammalian cells
  • 25. Preparation and assays for mammalian ISWI complexes
  • 26. Preparation and analysis of the INO80 complex
  • 27. Assay of Z-DNA induction by chromatin remodeling factors
  • 28. Immuno-depletion and purification strategies to study chromatin-remodeling factors in vitro
  • 29. Transcription through the nucleosome by mRNA-producing RNA polymerases
  • 30. Reconstitution and transcriptional analysis of chromatin in vitro
  • 31. Techniques used to study transcription on chromatin templates
  • 32. Analysis of DNA repair on nucleosome templates.
Description
xxxviii, 546 p., [7] p. of plates : ill. (some col.) ; 23 cm.
Notes
Includes bibliographical references and index.
Series Statement
Methods in enzymology 0076-6879 ; v. 377
Technical Details
  • Access in Virgo Classic

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