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Mapping Protein/DNA Interactions by Cross-Linking [electronic book]

INSERM
Format
EBook; Book; Online
Published
[Bethesda, MD : NCBI], 2001.
Language
English
Contents
  • Dissecting fission yeast centromeres via silencing
  • Localizing replication sties and nuclear proteins
  • Finding downstream target DNAs for chromatin proteins by X-CHIP in drosophila
  • Emerging techniques: UV laser footprinting and protein-DNA crosslinking
  • Nuclear organization and gene expression: visualization of transcription and higher order chromatin structure
  • Examining the distribution of telomeric and DNA repair proteins by ChrIP and real-time PCR
  • Nuclear organisation and gene silencing in lymphoctyes
  • Direct visualization of transcription factor binding to regulatory elements in living cells
  • Part I. Chromatin protein complexes: dynamic assemblies in the nucleus
  • Isolation of homeodomain engrailed target genes by UV cross-linking and chromatin IP
  • Analysis of chromatin assembly, chromatin domains, and DNA replication using xenopus systems
  • Binding sights in chromatin by X-ChIP
  • Chromatin immunoprecipitation in budding yeast
  • ChIP with native chromatin: advantages and problems relative to methods using cross-linked material
  • Highly sensitive FISH in combination with the visualization of the mammalian genome: architecture
  • Part II. Fly-FISHing: a protocol to localize single copy genes inside the nucleus in whole mount embryos
  • Part 2. Detailed method for immunoprecipitation of unfixed chromatin from mammalian cells.
Description
Mode of access: Internet.
Notes
  • Title from title screen (viewed November 20, 2003).
  • Includes bibliographical references.
Copyright Not EvaluatedCopyright Not Evaluated
Technical Details
  • Access in Virgo Classic
  • Staff View

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    a| Dissecting fission yeast centromeres via silencing -- Localizing replication sties and nuclear proteins -- Finding downstream target DNAs for chromatin proteins by X-CHIP in drosophila -- Emerging techniques: UV laser footprinting and protein-DNA crosslinking -- Nuclear organization and gene expression: visualization of transcription and higher order chromatin structure -- Examining the distribution of telomeric and DNA repair proteins by ChrIP and real-time PCR -- Nuclear organisation and gene silencing in lymphoctyes -- Direct visualization of transcription factor binding to regulatory elements in living cells -- Part I. Chromatin protein complexes: dynamic assemblies in the nucleus -- Isolation of homeodomain engrailed target genes by UV cross-linking and chromatin IP -- Analysis of chromatin assembly, chromatin domains, and DNA replication using xenopus systems -- Binding sights in chromatin by X-ChIP -- Chromatin immunoprecipitation in budding yeast -- ChIP with native chromatin: advantages and problems relative to methods using cross-linked material -- Highly sensitive FISH in combination with the visualization of the mammalian genome: architecture -- Part II. Fly-FISHing: a protocol to localize single copy genes inside the nucleus in whole mount embryos -- Part 2. Detailed method for immunoprecipitation of unfixed chromatin from mammalian cells.
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    a| DNA-protein interactions.
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    a| Proteins x| genetics.
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    a| DNA-Binding Proteins.
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