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Regulators and Effectors of Small GTPases: Part D Rho Family

edited by W. E. Balch, Channing J. Der, Alan Hall
Format
Book
Published
San Diego : Academic Press, c2000
Language
English
Related Title
Small GTPases and their regulators.
Series
Methods in Enzymology
ISBN
0121822265 (alk. paper)
Contents
  • Section I. Purification, Posttranslational Modification, and in Vitro Regulation
  • 1. Purification and Biochemical Characterization of TC10 / Cheryl L. Neudauer, Ian G. Macara 3
  • 2. Expression and Purification of Rho/RhoGDI Complexes / Paul W. Read, Robert K. Nakamoto 15
  • 3. Bacterial Expressed DH and DH/PH Domains / Kent L. Rossman, Sharon L. Campbell 25
  • 4. Biochemical Analysis of Regulation of Vav, a Guanine-Nucleotide Exchange Factor for Rho Family of GTPases / Raymond Mosteller, Jaewon Han, Balaka Das, Daniel Broek 38
  • 5. Activation of Rac1 by Human Tiam1 / Gideon Bollag, Anne M. Crompton, Danielle, Peverly-Mitchell, Gaston G. M. Habets, Marc Symons 51
  • 6. Activation of Rho GEF Activity by G[alpha subscript 13] / Matthew J. Hart, William Roscoe, Gideon Bollag 61
  • 7. Purification and Characterization of Guanine Nucleotide Dissociation Stimulator Protein / Jon P. Hutchinson, Katrin Rittinger, John F. Eccleston 71
  • 8. Purification and Biochemical Activity of Salmonella Exchange Factor SopE / Andrea Friebel, Wolf-Dietrich Hardt 82
  • 9. Stimulation of Rho GDI Release by ERM Proteins / Akiko Mammoto, Kazuo Takahashi, Takuya Sasaki, Yoshimi Takai 91
  • 10. Expression and Activity of Human Prenylcysteine-Directed Carboxyl Methyltransferase / Edwin Choy, Mark Philips 101
  • 11. Purification and Evaluation of Large Clostridial Cytotoxins That Inhibit Small GTPases of Rho and Ras Subfamilies / Michael Moos, Christoph von Eichel-Streiber 114
  • 12. Rho GTPase-Activating Toxins: Cytotoxic Necrotizing Factors and Dermonecrotic Toxin / Gudula Schmidt, Klaus Aktories 125
  • Section II. Purification and Activity of GTPase Targets
  • 13. Measurement of GTPase Effector Affinities / Danny Manor 139
  • 14. Purification and in Vitro Activity of Rho-Associated Kinase / Mutsuki Amano, Yuko Fukata, Hiroaki Shimokawa, Kozo Kaibuchi 149
  • 15. Purification and in Vitro Activities of p21-Activated Kinases / Charles C. King, Abina M. Reilly, Ulla G. Knaus 155
  • 16. Stimulation of Phospholipase C-[beta subscript 2] by Rho GTPases / Daria Illenberger, Ilona Stephan, Peter Gierschik, Frieder Schwald 167
  • 17. Regulation of Phospholipase D1 Activity by Rho GTPases / Michael A. Frohman, Yasunori Kanaho, Yue Zhang, Andrew J. Morris 177
  • 18. In Vitro Interaction of Phosphoinositide-4-phosphate 5-Kinases with Rac / Kimberley Tolias, Christopher L. Carpenter 190
  • 19. Activity and Regulation of p35/Cdk5 Kinase Complex / Margareta Nikolic, Li-Huei Tsai 200
  • 20. Actin Assembly Mediated by Arp2/3 Complex and WASP Family Proteins / R. Dyche Mullins, Laura M. Machesky 214
  • 21. In Vitro Actin Polymerization Using Polymorphonuclear Leukocyte Extracts / Sally H. Zigmond 237
  • Section III. Analysis of Rho GTPase Function
  • 22. Determination of GTP Loading on Rac and Cdc42 in Platelets and Fibroblasts / Anser C. Azim, Kurt L. Barkalow, John H. Hartwig 257
  • 23. Determination of GTP Loading on Rho / Xiang-Dong Ren, Martin Alexander Schwartz 264
  • 24. Use and Properties of ROCK-Specific Inhibitor Y-27632 / Shuh Narumiya, Toshimasa Ishizaki, Masayoshi Uehata 273
  • 25. Inducible Membrane Recruitment of Small GTP-Binding Proteins by Rapamycin-Based System in Living Cells / Flavia Castellano, Philippe Chavrier 285
  • 26. Expression of Rho GTPases Using Retroviral Vectors / Frits Michiels, Rob A. van der Kammen, Lennert Janssen, Garry Nolan, John G. Collard 295
  • 27. Expression of Rho GTPases Using Adenovirus Vectors / Daniel M. Sullivan, Toren Finkel 303
  • 28. In Vivo Activity of Wild-Type and Mutant PAKs / Charles C. King, Luraynne C. Sanders, Gary M. Bokoch 315
  • 29. Single Cell Assays for Rac Activity / Laura J. Taylor, Amy B. Walsh, Patrick Hearing, Dafna Bar-Sagi 327
  • 30. Effect of Rho GTPases on Na-H Exchanger in Mammalian Cells / Sheryl P. Denker, Weihong Yan, Diane L. Barber 334
  • 31. Actin Filamenti Assembly in Permeabilized Platelets / John H. Hartwig, Kurt L. Barkalow 349
  • 32. Rho GTPases: Secretion and Actin Dynamics in Permeabilized Mast Cells / Mark R. Holt, Anna Koffer 356
  • 33. Isolation and in Vitro Contraction of Stress Fibers / Kazuo Katoh, Yumiko Kano, Keigi Fujiwara 369
  • 34. Inhibition of Rho GTPases Using Protein Geranylgeranyltransferase I Inhibitors / Said M. Sebti, Andrew D. Hamilton 381
  • 35. Imaging Spatiotemporal Dynamics of Rac Activation in Vivo with FLAIR / Chester E. Chamberlain, Vadim S. Kraynov, Klaus M. Hahn 389
  • Section IV. Biological Assays of Rho GTPase Function
  • 36. Rho-like GTPases in Tumor Cell Invasion / Eva E. Evers, Rob A. van der Kammen, Jean P. ten Klooster, John G. Collard 403
  • 37. Mouse Embryo Fibroblasts: A Genetic Model System for Studying Rho- and Ras-Dependent Cell Cycle Progression / Giovanna M. D'Abaco, Michael F. Olson 415
  • 38. Analyses of Transforming Activity of Rho Family Activators / Patricia A. Solski, Karon Abe, Channing J. Der 425
  • 39. Rho GTPases and Cell Migration-Fibroblast Wound Healing / Catherine D. Nobes 441
  • 40. Rho GTPases and Cell Migration: Measurement of Macrophage Chemotaxis / Gareth E. Jones, Anne J. Ridley, Daniel Zicha 449
  • 41. Rho GTPases and Macrophage Phagocytosis / Jayesh C. Patel, Alan Hall, Emmanuelle Caron 462
  • 42. Rho GTPases and Axonal Growth Cone Collapse / Alyson E. Fournier, Robert G. Kalb, Stephen M. Strittmatter 473
  • 43. Study of in Situ Function of Cytoskeletal Proteins in Lamellipodia and Filopodia Using Microscale Chromophore-Assisted Laser Inactivation / Eric V. Wong, Daniel G. Jay 482
  • 44. Modulation of Actin Cytoskeleton by Salmonella GTPase Activating Protein SptP / Jorge E. Galan, Yixin Fu 496.
Description
xxxv,547 p., [1] leaf of plates : ill. (some col.) ; 24 cm.
Notes
  • Update of: Small GTPases and their regulators. Part B, Rho family (Methods in enzymology ; v. 255).
  • Includes bibliographical references and indexes.
Series Statement
Methods in enzymology 0076-6879 ; v. 325
Technical Details
  • Access in Virgo Classic
  • Staff View

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    g| Section I. t| Purification, Posttranslational Modification, and in Vitro Regulation -- g| 1. t| Purification and Biochemical Characterization of TC10 / r| Cheryl L. Neudauer, Ian G. Macara g| 3 -- g| 2. t| Expression and Purification of Rho/RhoGDI Complexes / r| Paul W. Read, Robert K. Nakamoto g| 15 -- g| 3. t| Bacterial Expressed DH and DH/PH Domains / r| Kent L. Rossman, Sharon L. Campbell g| 25 -- g| 4. t| Biochemical Analysis of Regulation of Vav, a Guanine-Nucleotide Exchange Factor for Rho Family of GTPases / r| Raymond Mosteller, Jaewon Han, Balaka Das, Daniel Broek g| 38 -- g| 5. t| Activation of Rac1 by Human Tiam1 / r| Gideon Bollag, Anne M. Crompton, Danielle, Peverly-Mitchell, Gaston G. M. Habets, Marc Symons g| 51 -- g| 6. t| Activation of Rho GEF Activity by G[alpha subscript 13] / r| Matthew J. Hart, William Roscoe, Gideon Bollag g| 61 -- g| 7. t| Purification and Characterization of Guanine Nucleotide Dissociation Stimulator Protein / r| Jon P. Hutchinson, Katrin Rittinger, John F. Eccleston g| 71 -- g| 8. t| Purification and Biochemical Activity of Salmonella Exchange Factor SopE / r| Andrea Friebel, Wolf-Dietrich Hardt g| 82 -- g| 9. t| Stimulation of Rho GDI Release by ERM Proteins / r| Akiko Mammoto, Kazuo Takahashi, Takuya Sasaki, Yoshimi Takai g| 91 -- g| 10. t| Expression and Activity of Human Prenylcysteine-Directed Carboxyl Methyltransferase / r| Edwin Choy, Mark Philips g| 101 -- g| 11. t| Purification and Evaluation of Large Clostridial Cytotoxins That Inhibit Small GTPases of Rho and Ras Subfamilies / r| Michael Moos, Christoph von Eichel-Streiber g| 114 -- g| 12. t| Rho GTPase-Activating Toxins: Cytotoxic Necrotizing Factors and Dermonecrotic Toxin / r| Gudula Schmidt, Klaus Aktories g| 125 -- g| Section II. t| Purification and Activity of GTPase Targets -- g| 13. t| Measurement of GTPase Effector Affinities / r| Danny Manor g| 139 -- g| 14. t| Purification and in Vitro Activity of Rho-Associated Kinase / r| Mutsuki Amano, Yuko Fukata, Hiroaki Shimokawa, Kozo Kaibuchi g| 149 -- g| 15. t| Purification and in Vitro Activities of p21-Activated Kinases / r| Charles C. King, Abina M. Reilly, Ulla G. Knaus g| 155 -- g| 16. t| Stimulation of Phospholipase C-[beta subscript 2] by Rho GTPases / r| Daria Illenberger, Ilona Stephan, Peter Gierschik, Frieder Schwald g| 167 -- g| 17. t| Regulation of Phospholipase D1 Activity by Rho GTPases / r| Michael A. Frohman, Yasunori Kanaho, Yue Zhang, Andrew J. Morris g| 177 -- g| 18. t| In Vitro Interaction of Phosphoinositide-4-phosphate 5-Kinases with Rac / r| Kimberley Tolias, Christopher L. Carpenter g| 190 -- g| 19. t| Activity and Regulation of p35/Cdk5 Kinase Complex / r| Margareta Nikolic, Li-Huei Tsai g| 200 -- g| 20. t| Actin Assembly Mediated by Arp2/3 Complex and WASP Family Proteins / r| R. Dyche Mullins, Laura M. Machesky g| 214 -- g| 21. t| In Vitro Actin Polymerization Using Polymorphonuclear Leukocyte Extracts / r| Sally H. Zigmond g| 237 -- g| Section III. t| Analysis of Rho GTPase Function -- g| 22. t| Determination of GTP Loading on Rac and Cdc42 in Platelets and Fibroblasts / r| Anser C. Azim, Kurt L. Barkalow, John H. Hartwig g| 257 -- g| 23. t| Determination of GTP Loading on Rho / r| Xiang-Dong Ren, Martin Alexander Schwartz g| 264 -- g| 24. t| Use and Properties of ROCK-Specific Inhibitor Y-27632 / r| Shuh Narumiya, Toshimasa Ishizaki, Masayoshi Uehata g| 273 -- g| 25. t| Inducible Membrane Recruitment of Small GTP-Binding Proteins by Rapamycin-Based System in Living Cells / r| Flavia Castellano, Philippe Chavrier g| 285 -- g| 26. t| Expression of Rho GTPases Using Retroviral Vectors / r| Frits Michiels, Rob A. van der Kammen, Lennert Janssen, Garry Nolan, John G. Collard g| 295 -- g| 27. t| Expression of Rho GTPases Using Adenovirus Vectors / r| Daniel M. Sullivan, Toren Finkel g| 303 -- g| 28. t| In Vivo Activity of Wild-Type and Mutant PAKs / r| Charles C. King, Luraynne C. Sanders, Gary M. Bokoch g| 315 -- g| 29. t| Single Cell Assays for Rac Activity / r| Laura J. Taylor, Amy B. Walsh, Patrick Hearing, Dafna Bar-Sagi g| 327 -- g| 30. t| Effect of Rho GTPases on Na-H Exchanger in Mammalian Cells / r| Sheryl P. Denker, Weihong Yan, Diane L. Barber g| 334 -- g| 31. t| Actin Filamenti Assembly in Permeabilized Platelets / r| John H. Hartwig, Kurt L. Barkalow g| 349 -- g| 32. t| Rho GTPases: Secretion and Actin Dynamics in Permeabilized Mast Cells / r| Mark R. Holt, Anna Koffer g| 356 -- g| 33. t| Isolation and in Vitro Contraction of Stress Fibers / r| Kazuo Katoh, Yumiko Kano, Keigi Fujiwara g| 369 -- g| 34. t| Inhibition of Rho GTPases Using Protein Geranylgeranyltransferase I Inhibitors / r| Said M. Sebti, Andrew D. Hamilton g| 381 -- g| 35. t| Imaging Spatiotemporal Dynamics of Rac Activation in Vivo with FLAIR / r| Chester E. Chamberlain, Vadim S. Kraynov, Klaus M. Hahn g| 389 -- g| Section IV. t| Biological Assays of Rho GTPase Function -- g| 36. t| Rho-like GTPases in Tumor Cell Invasion / r| Eva E. Evers, Rob A. van der Kammen, Jean P. ten Klooster, John G. Collard g| 403 -- g| 37. t| Mouse Embryo Fibroblasts: A Genetic Model System for Studying Rho- and Ras-Dependent Cell Cycle Progression / r| Giovanna M. D'Abaco, Michael F. Olson g| 415 -- g| 38. t| Analyses of Transforming Activity of Rho Family Activators / r| Patricia A. Solski, Karon Abe, Channing J. Der g| 425 -- g| 39. t| Rho GTPases and Cell Migration-Fibroblast Wound Healing / r| Catherine D. Nobes g| 441 -- g| 40. t| Rho GTPases and Cell Migration: Measurement of Macrophage Chemotaxis / r| Gareth E. Jones, Anne J. Ridley, Daniel Zicha g| 449 -- g| 41. t| Rho GTPases and Macrophage Phagocytosis / r| Jayesh C. Patel, Alan Hall, Emmanuelle Caron g| 462 -- g| 42. t| Rho GTPases and Axonal Growth Cone Collapse / r| Alyson E. Fournier, Robert G. Kalb, Stephen M. Strittmatter g| 473 -- g| 43. t| Study of in Situ Function of Cytoskeletal Proteins in Lamellipodia and Filopodia Using Microscale Chromophore-Assisted Laser Inactivation / r| Eric V. Wong, Daniel G. Jay g| 482 -- g| 44. t| Modulation of Actin Cytoskeleton by Salmonella GTPase Activating Protein SptP / r| Jorge E. Galan, Yixin Fu g| 496.
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