Item Details

Molecular Embryology: Methods and Protocols

edited by Paul T. Sharpe and Ivor Mason
Format
Book
Published
Totowa, N.J. : Humana Press, c1999.
Language
English
Series
Methods in Molecular Biology
Methods in Molecular Biology (Clifton, N.J.)
ISBN
0896033872 (alk. paper)
Contents
  • Pt.1. The mouse embryo
  • The mouse as a developmental model
  • Culture of postimplantation mouse embryos
  • Organ culture in the analysis of tissue interactions
  • Treatment of mice with retinoids in vivo and in vitro: skeletal staining
  • Cell grafting and labeling in postimplantation mouse embryos
  • Production of transgenic rodents by the microinjection of coloned DNA into fertilized one-celled eggs
  • Cre recombinase mediated alterations of the mouse genome using embryonic stem cells
  • Gene trapping in mouse embryonic stem cells
  • Production and growth of conditionally immortal cell lines from the H-2KbtsA58 transgenic mouse
  • Reporter genes for the study of transcriptional regulation in transgenic mouse embryos
  • Application of IacZ transgenic mice to cell lineage studies
  • Mouse primordial germ cells: isolation and in vitro cultue
  • Pt.2. The chicken embryo
  • The avian embryo: an overview
  • Chick embryos: incubation and isolation
  • New culture
  • Grafting Hensen's node
  • Grafting of Somites
  • Notochord grafts
  • Transplantation of avian neural tissue
  • Grafting of apical ridge and polarizing region
  • - Tissue recombinations in collagen gels
  • Quail-chick chimeras
  • Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo
  • Pt.3. Amphibian embryo
  • An overview of Xenopus development
  • Mesoderm induction assays
  • Experimental embryological methods for analysis of neural induction in the amphibian
  • A method for generating transgenic frog embryos
  • Axolotl/newt
  • Pt.4. Zebrafish
  • Pt.5. Nonvertebrate chordates
  • Pt.6. Retroviruses
  • Pt.7. Molecular techniques
  • Pt.8. Microscopy and photography.
Description
xvi, 756 p., [8] p. of plates : ill. (some col.) ; 24 cm.
Notes
Includes bibliographical references and index.
Series Statement
Methods in molecular biology ; 97
Methods in molecular biology (Clifton, N.J.) v. 97
Technical Details
  • Access in Virgo Classic

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    a| Molecular embryology : b| methods and protocols / c| edited by Paul T. Sharpe and Ivor Mason.
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    a| Totowa, N.J. : b| Humana Press, c| c1999.
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    a| xvi, 756 p., [8] p. of plates : b| ill. (some col.) ; c| 24 cm.
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    a| Methods in molecular biology ; v| 97
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    a| Includes bibliographical references and index.
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    a| Pt.1. The mouse embryo -- The mouse as a developmental model -- Culture of postimplantation mouse embryos -- Organ culture in the analysis of tissue interactions -- Treatment of mice with retinoids in vivo and in vitro: skeletal staining -- Cell grafting and labeling in postimplantation mouse embryos -- Production of transgenic rodents by the microinjection of coloned DNA into fertilized one-celled eggs -- Cre recombinase mediated alterations of the mouse genome using embryonic stem cells -- Gene trapping in mouse embryonic stem cells -- Production and growth of conditionally immortal cell lines from the H-2KbtsA58 transgenic mouse -- Reporter genes for the study of transcriptional regulation in transgenic mouse embryos -- Application of IacZ transgenic mice to cell lineage studies -- Mouse primordial germ cells: isolation and in vitro cultue -- Pt.2. The chicken embryo -- The avian embryo: an overview -- Chick embryos: incubation and isolation -- New culture -- Grafting Hensen's node -- Grafting of Somites -- Notochord grafts -- Transplantation of avian neural tissue -- Grafting of apical ridge and polarizing region --- Tissue recombinations in collagen gels -- Quail-chick chimeras -- Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo -- Pt.3. Amphibian embryo -- An overview of Xenopus development -- Mesoderm induction assays -- Experimental embryological methods for analysis of neural induction in the amphibian -- A method for generating transgenic frog embryos -- Axolotl/newt -- Pt.4. Zebrafish -- Pt.5. Nonvertebrate chordates -- Pt.6. Retroviruses -- Pt.7. Molecular techniques -- Pt.8. Microscopy and photography.
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    a| Mice.
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    a| Amphibians
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    a| Zebrafish.
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    a| Chordata, Nonvertebrate.
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    a| Sharpe, Paul T.
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    a| Mason, Ivor.
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    a| Methods in molecular biology (Clifton, N.J.) v| v. 97.
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