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Resin Microscopy and on-Section Immunocytochemistry

G.R. Newman, J.A. Hobot
Format
Book
Published
Berlin ; New York : Springer-Verlag, c1993.
Language
English
Series
Springer Laboratory
ISBN
3540564292 (Berlin : alk. paper), 0387564292 (New York : alk. paper)
Contents
  • Resin embedding
  • Strategic approach
  • Resins
  • Epoxy resins
  • Acrylic resins
  • Resin embedding protocols for chemically fixed tissue
  • Tissue handling
  • Protocols employing full dehydration of tissue at room temperature (RT)
  • Protocols employing partial dehydration of tissue at room temperature (RT)
  • Protocols employing dehydration of tissue at cold temperatures (0⁰C to -20⁰C)
  • Protocols employing dehydration of tissue at progressively lower temperatures (PLT: -35⁰C to -50⁰C)
  • Cryotechniques
  • Tissue preparation for freezing
  • Rapid freezing
  • Cryosubstitution
  • Protocols for cryosubstitution (epoxy resins)
  • Protocols for cryosubstitution (acrylic resins)
  • Freeze-drying
  • Protocols for freeze-drying (epoxy resins)
  • Protocols for freeze-drying (acrylic resins)
  • Cryoultramicrotomy
  • Methods for resin polymerisation
  • Heat polymerisation methods
  • Chemical catalytic polymerisation methods
  • Ultraviolet light polymerisation methods
  • Uncatalysed LR white
  • Handling resin blocks
  • Sectioning blocks
  • Storing blocks
  • On-section immunolabelling.
  • (cont) Strategies in immunolabelling
  • Colloidal gold strategies
  • Peroxidase strategies
  • EM double immunolabelling
  • General considerations
  • Resin section pretreatment
  • Resin section immunolabelling
  • Controls in immunocytochemistry
  • Immunolabelling protocols for resin sections
  • Pretreatment protocols
  • Immunocolloidal gold labelling protocols
  • Immunoperoxidase labelling protocols
  • EM double immunolabelling protocols
  • Immunocolloidal gold labelling protocols for cryoultramicrotomy
  • Resin embedding and immunolabelling
  • Penetration of label
  • Resin cross-linking
  • Surface relief of sections
  • Quantitation
  • Examples of typical resin embedding regimes for immunocytochemistry.
Description
xix, 221 p. : ill. ; 25 cm.
Notes
Includes bibliographical references (p. [197]-212) and index.
Local Notes
HathiTrust shared print program 2017.
Technical Details
  • Access in Virgo Classic
  • Staff View

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    a| Newman, G. R. q| (Geoffrey R.), d| 1943-
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    a| Resin microscopy and on-section immunocytochemistry / c| G.R. Newman, J.A. Hobot.
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    a| Berlin ; a| New York : b| Springer-Verlag, c| c1993.
    300
      
      
    a| xix, 221 p. : b| ill. ; c| 25 cm.
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    a| Springer laboratory
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    a| Includes bibliographical references (p. [197]-212) and index.
    505
    0
      
    a| Resin embedding -- Strategic approach -- Resins -- Epoxy resins -- Acrylic resins -- Resin embedding protocols for chemically fixed tissue -- Tissue handling -- Protocols employing full dehydration of tissue at room temperature (RT) -- Protocols employing partial dehydration of tissue at room temperature (RT) -- Protocols employing dehydration of tissue at cold temperatures (0⁰C to -20⁰C) -- Protocols employing dehydration of tissue at progressively lower temperatures (PLT: -35⁰C to -50⁰C) -- Cryotechniques -- Tissue preparation for freezing -- Rapid freezing -- Cryosubstitution -- Protocols for cryosubstitution (epoxy resins) -- Protocols for cryosubstitution (acrylic resins) -- Freeze-drying -- Protocols for freeze-drying (epoxy resins) -- Protocols for freeze-drying (acrylic resins) -- Cryoultramicrotomy -- Methods for resin polymerisation -- Heat polymerisation methods -- Chemical catalytic polymerisation methods -- Ultraviolet light polymerisation methods -- Uncatalysed LR white -- Handling resin blocks -- Sectioning blocks -- Storing blocks -- On-section immunolabelling.
    505
    0
      
    a| (cont) Strategies in immunolabelling -- Colloidal gold strategies -- Peroxidase strategies -- EM double immunolabelling -- General considerations -- Resin section pretreatment -- Resin section immunolabelling -- Controls in immunocytochemistry -- Immunolabelling protocols for resin sections -- Pretreatment protocols -- Immunocolloidal gold labelling protocols -- Immunoperoxidase labelling protocols -- EM double immunolabelling protocols -- Immunocolloidal gold labelling protocols for cryoultramicrotomy -- Resin embedding and immunolabelling -- Penetration of label -- Resin cross-linking -- Surface relief of sections -- Quantitation -- Examples of typical resin embedding regimes for immunocytochemistry.
    590
      
      
    a| HathiTrust shared print program 2017.
    596
      
      
    a| 14
    650
      
    0
    a| Immunocytochemistry x| Technique.
    650
      
    0
    a| Electron microscopy x| Technique.
    650
      
    0
    a| Gums and resins.
    650
      
    2
    a| Acrylic Resins.
    650
      
    2
    a| Epoxy Resins.
    650
      
    2
    a| Immunohistochemistry.
    650
      
    2
    a| Microscopy, Electron.
    650
      
    2
    a| Tissue Embedding.
    653
    0
      
    a| Humans a| Tissues a| Microscopy
    700
    1
      
    a| Hobot, J. A. q| (Jan A.), d| 1950-
    019
      
      
    a| 29029078
    069
    0
      
    a| 9309483
    590
      
      
    a| HathiTrust shared print program 2017.
    999
      
      
    a| QR187 .I45 N48 1993 w| LC i| X002758739 l| BY-REQUEST m| IVY t| BOOK
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