Item Details

Print View

Ultrasound-Aided Multi-Parametric Photoacoustic Microscopy of the Mouse Brain

Ning, Bo; Hu, Song; Sun, Naidi; Cao, Rui; Hossack, John
Format
Article
Author
Ning, Bo
Hu, Song
Sun, Naidi
Cao, Rui
Hossack, John
Abstract
High-resolution quantitative imaging of cerebral oxygen metabolism in mice is crucial for understanding brain functions and formulating new strategies to treat neurological disorders, but remains a challenge. Here, we report on our newly developed ultrasound-aided multi-parametric photoacoustic microscopy (PAM), which enables simultaneous quantification of the total concentration of hemoglobin (CHb), the oxygen saturation of hemoglobin (sO2), and cerebral blood flow (CBF) at the microscopic level and through the intact mouse skull. The three-dimensional skull and vascular anatomies delineated by the dual-contrast (i.e., ultrasonic and photoacoustic) system provide important guidance for dynamically focused contour scan and vessel orientation-dependent correction of CBF, respectively. Moreover, bi-directional raster scan allows determining the direction of blood flow in individual vessels. Capable of imaging all three hemodynamic parameters at the same spatiotemporal scale, our ultrasound-aided PAM fills a critical gap in preclinical neuroimaging and lays the foundation for high-resolution mapping of the cerebral metabolic rate of oxygen (CMRO2)—a quantitative index of cerebral oxygen metabolism. This technical innovation is expected to shed new light on the mechanism and treatment of a broad spectrum of neurological disorders, including Alzheimer’s disease and ischemic stroke.
Language
English
Date Received
2017-08-30
Published
University of Virginia, 2017
Published Date
2017-08-30
Collection
Libra Open Repository
Related Resources
https://www.nature.com/articles/srep18775
In CopyrightIn Copyright
▾See more
▴See less

Availability

Read Online