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Different Fates of Alzheimer's Disease Amyloid-Beta Fibrils Remodeled by Biocompatible Small Molecules

Irwin, Jacob
Thesis/Dissertation; Online
Irwin, Jacob
Kwon, Inchan
Amyloid fibril protein aggregates implicated in numerous human diseases are thermodynamically very stable. Stringent conditions that would not be possible in a physiological environment are often required to disrupt the stable fibrils. Recently, there is increasing evidence that small molecules can remodel amyloid fibrils in a physiologically relevant manner. In order to investigate possible fibril remodeling mechanisms using this approach, we performed comparative studies on the structural features of the different amyloid-beta (Aβ) aggregates remodeled from Aβ fibrils by three biocompatible small molecules: methylene blue (MB); brilliant blue G (BBG); and erythrosine B (ER). Combined with circular dichroism (CD), immuno-blotting, transmission electron microscopy (TEM), and atomic force microscopy (AFM) results, it was found that brilliant blue G- and erythrosine B-treatment generate fragmented Aβ fibrils and protofibrils, respectively. In contrast, incubation of the Aβ fibrils with methylene blue perturbs fibrillar structure leading to amorphous Aβ aggregates. These findings provide insights on the molecular mechanism of amyloid fibril formation and remodeling and also illustrate the possibility of controlled changes in biomolecule nanostructures.
University of Virginia, Department of Chemical Engineering, MS (Master of Science), 2013
Published Date
MS (Master of Science)
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